DEVELOPMENT OF NANOSENSORS FOR DETERMINING LIPIDS IN BOVINE MILK
dairy fat; RGB; spectroscopy; gold nanoparticle; 10,12-pentacosadinoic acid
Milk is a highly nutritious biofluid, widely consumed in the world, capable of meeting the basic needs of individuals. Brazil ranks third in world production of bovine milk. The composition of milk, in particular the fat content, is an essential factor for the final quality of products, in addition to significantly affecting prices for producers. Dairy fats are composed of fatty acids, especially palmitic acid (C:16) and oleic acid (C:18:1), which are responsible for the rheological and organoleptic characteristics of milk and its by-products. Therefore, the quantification of fat in bovine milk is extremely important for the dairy industry, and currently the Gerber method is the most used, however they have a large margin of error and are dangerous. We present a colorimetric methodology for the interaction of gold nanoparticles and 10,12-pentacosadiinoic acid with palmitic acid and oleic acid. The technique was validated by UV-Vis spectroscopy and the RGB color methodology, showing excellent results, in which it was possible to verify the color change of the samples as the concentration of fatty acid increased. This is a promising study for the production of sensitive, low-cost and selective colorimetric nanosensors of dairy fat.