Banca de DEFESA: BRUNA PALOMA RIBEIRO

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : BRUNA PALOMA RIBEIRO
DATE: 22/12/2022
TIME: 14:00
LOCAL: Pelo link: https://meet.google.com/pfb-kjqd-yav
TITLE:

IMMOBILIZATION OF MICROORGANISMS IN CARRAGEENA SPHERES

KEY WORDS:

carrageenan sphere; immobilization of microorganisms; yeasts; fermentation.

PAGES: 60
BIG AREA: Engenharias
AREA: Engenharia Química
SUBÁREA: Processos Industriais de Engenharia Química
SUMMARY:

 

The present work aimed to synthesize carrageenan spheres and to immobilize microorganisms in their polymeric structure. Two yeasts Saccharomyces pastorianus and Saccharomyces cerevisiae with immobilized microorganisms were chosen. The immobilized yeasts were tested in fermentation using three different substrates: brown sugar, crystal sugar and D(+)sucrose. The synthesis of the carrageenan sphere was achieved when there was a combination of the concentrations of two solutions: the carrageenan solution, at a concentration of 10 g.L-1, and the KCl solution, at a concentration of 0.848 mol. L-1. At these same concentrations, it was possible to immobilize the yeasts in the carrageenan polymeric matrix and form spheres. The results of the fermentations with immobilized yeasts show that the consumption of sugar occurs by two factors: by the adsorption of sugar in the polymeric matrix of the carrageenan, due to the electrostatic interactions between them and also by the consumption of sugar by the immobilized yeasts. Sucrose was the best sugar to use as a substrate. Regarding the microorganisms, it can be noted that Saccharomyces cerevisiae obtained more satisfactory results, due to the greater speed and greater consumption of sugar, in comparison with the other yeast. This higher consumption can be explained by the greater amount of viable cells present within the spheres. In addition, the spheres were dried with immobilized yeasts in the desiccator, in an oven and naturally. However, the cells after rehydration of the beads are not viable. In view of all this, it can be concluded that microorganism cells can be immobilized in carrageenan spheres, however, the immobilization process must be refined to increase cell viability. In addition, immobilized cells can be used and reused in biotechnological processes to produce products of interest.

BANKING MEMBERS:
Presidente - 1810291 - DEMIAN PATRICK FABIANO
Interno - 1811284 - EDSON ROMANO NUCCI
Interno - 1742695 - JUAN CANELLAS BOSCH NETO
Externa à Instituição - CÍNTHIA MARIA FELÍCIO - IF-GOIANO