Banca de DEFESA: ARTHUR CARVALHO PIRES

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : ARTHUR CARVALHO PIRES
DATE: 11/02/2022
TIME: 14:00
LOCAL: LANEC/CDB/UFSJ
TITLE:

DEVELOPMENT OF AN AUTOMATED SYSTEM FOR THE CONTROL OF FLOW AND EXCHANGE OF SOLUTIONS IN ELECTROPHYSIOLOGICAL REGISTRATION SYSTEMS OF HIPOCAMPAAL SLICES.

KEY WORDS:

Keywords: Electrophysiology, Flow Control, Exchange of Solutions, Perfusion

PAGES: 35
BIG AREA: Multidisciplinar
AREA: Interdisciplinar
SUMMARY:

Gravity perfusion systems have a flow dependent on the difference between the levels of the supply reservoir and the infusion point. In electrophysiology experiments in which such systems are used, it is usual to periodically adjust the flow and exchange solutions manually. However, the handling of regulators for flow control, and manual valves for changing solutions during signal acquisition can cause mechanical and electrical interference from different sources, impairing the signal/noise ratio. This work describes the materials and procedures used in the construction of an automatic flow control system with gradual adjustments and exchange of perfusion solutions in electrophysiological experiments with brain slices. And it aims to reduce, as much as possible, the artifacts originating from the procedures for closing and opening records of each solution used. An optical sensor for drop instrumentation and two flow actuating devices were developed. The exchange device consists of solenoid valves and pumps to recirculate the solution. A Human Machine Interface (HMI) was developed for user interaction with the system, and an Atmel2550 microcontroller was used as the control unit for the entire set. All manufactured devices were tested in electrophysiological recording systems for the acquisition of electrical signals in hippocampal slices through an interface chamber. The system proved to be an effective tool for the standardization of experiments, in addition, the results also showed a decrease in flow error and attenuation of interferences in the procedures for exchanging perfusion solutions.

BANKING MEMBERS:
Presidente - 6434894 - ANTONIO CARLOS GUIMARAES DE ALMEIDA
Interno - 075.270.776-03 - LUIZ EDUARDO CANTON SANTOS - IPTAN
Interno - 2487650 - ANTONIO MARCIO RODRIGUES
Externo à Instituição - DAMIEN THOMAS DEPANNEMAECKER - CNRS