Disertación/Tesis
2024
Disertaciones
1
  • PATRICIA ALVES TORGA
  • EXPRESSION OF NON-SYNAPTIC MECHANISM IN INTERNEURONS ASSOCIATED WITH SPONTANEOUS CRISES PROFILE IN GERBILS

  • Líder : ANTONIO CARLOS GUIMARAES DE ALMEIDA
  • MIEMBROS DE LA BANCA :
  • ANTONIO CARLOS GUIMARAES DE ALMEIDA
  • LUIZ EDUARDO CANTON SANTOS
  • ANTONIO MARCIO RODRIGUES
  • SERGIO GOMES DA SILVA
  • Data: 10-ene-2024


  • Resumen Espectáculo
  • Mongolian gerbils are experimental models of great relevance for the study of
    epileptogenesis. Adult animals, when subjected to stress-inducing maneuvers, present
    spontaneous crises. Unlike those belonging to a young group, who do not present
    crises. A comparative analysis between the two groups is very useful. Adult animals
    present different profiles of resistance and sensitivity to crisis-inducing maneuvers. In
    work conducted by Aarão (2022), at LANEC, using experimental conditions of synapse
    suppression, statistically significant variations were observed in the electrographic
    recordings obtained from hippocampal slices from groups of young pre-epileptic gerbils
    (Pep), highly sensitive adults (HSS) and resistant (SR), subdivided into temporal
    scales after crisis induction. In addition, immunohistochemical expression analyses of
    PV, NKCC1, KCC2, NHE1, Na+/K+-ATPase, GAD65/67 and Cx36 were performed in
    hippocampal slices from the gerbils groups and no statistically significant differences
    were observed in relation to the electrographic variations found, but a numerical
    superiority of PV- immunoreactive interneurons was observed in the PEp gerbils group.
    Considering the absence of seizures in this age group and the importance of this cell
    subpopulation in the inhibitory control of the neural network, it was proposed, for the
    present study, to further investigate the expression of PV, NKCC1, KCC2, Na+/K+-
    ATPase and Cx36 in these interneurons. To carry out this work, it was used the slide
    bank made by Aarão (2022), from which the PV-immunoreactive interneurons and
    morphological like PV-immunoreactive interneurons (MLPVI) were identified, isolated,
    and immunohistochemical analysis were performed for the proteins of interest, using
    optical densitometry. The expression of proteins observed in PV-immunoreactive
    interneurons of PEp gerbils was consistent with the electrographic findings obtained
    by Aarão (2022), since it favors the occurrence of ionic microenvironments that can
    lead to the deflagration of depolarizing potentials that propagate and sustain
    themselves in the neural network, synchronously recruiting a large population of
    pyramidal cells. Thus, under conditions of synapse suppression, the protein expression
    profile observed in PV-immunoreactive interneurons is compatible with the tendency
    to synchronize ictal events in PEp gerbils, as observed in the electrographic
    recordings. In vivo, the higher expression of PV in this group suggests its essential
    role in the generation of effective IPSPs in the neural network, preventing the
    occurrence of seizures in this age group.

2
  • GABRIELA STEFANE BARBOSA MENDES
  • Identification of new genomics region, genes and allele that confer Al tolerance in tropical maize

  • Líder : CLAUDIA TEIXEIRA GUIMARAES
  • MIEMBROS DE LA BANCA :
  • CLAUDIA TEIXEIRA GUIMARAES
  • JURANDIR VIEIRA DE MAGALHÃES
  • KAIO OLIMPIO DAS GRAÇAS DIAS
  • Data: 26-feb-2024


  • Resumen Espectáculo
  • Maize is a worldwide crop, but its cultivation in acidic soils causes significant yield losses. Acid soils show ionic forms of aluminum (Al), which limit the development of the root system, harming the absorption of water and mineral nutrients. In the present work, a new population of 280 F2 individuals compiled from the cross between L228-3 (moderately Al-tolerant line) and L53 (Al-sensitive line) was used to map genomic regions associated with Al tolerance. F2 individuals were phenotyped for Al tolerance in hydroponics, with the susceptibility based on the inhibition of root growth by Al, and genotyped for SNP (Single Nucleotide Polymorphisms) markers and for an allele-specific marker for the ZmMATE1 gene, which confers Al tolerance in maize. The genetic map was composed of 477 Mendelian markers, covering 1152.95 cM throughout 10 chromosomes. The multiple interval mapping approach allowed us to detect five genomic regions associated with Al tolerance on chromosomes 1, 5, 6, 7, 9, and three epistatic interactions, explaining 47.8% of Al tolerance. The QTL with the greatest effect was found on chromosome 6 (qALT6), co-located with the ZmMATE1 gene, and explained 24.9% of Al tolerance. The expression pattern confirmed that the ZmMATE1 gene was induced by Al in the root apex of the L228-3 line, in a similar way found in the Cateto Al237 line. However, just one copy of ZmMATE1 in L228-3, against the three copies found in Cateto Al237, suggests that L228-3 has a new allele for the ZmMATE1 gene. The second QTL, in terms of effect, was mapped on chromosome 9 (qALT9), explained 7.5% of Al tolerance, and showed an epistatic interaction with qALT6. In the qALT9 region, we found genes encoding transcription factors of the MYB, NAC, and WRKY types. Only the transcription factor MYB, among those genes, was induced by Al after one hour of exposure to Al in the L53 line. This result, more the signal of the effect on tolerance and in silico evidence of the presence of a binding site for MYB in the promoter region of the ZmMATE1 gene, suggests that the MYB family transcription factor acts to control the expression of the ZmMATE1 gene. However, further functional studies are still needed to validate this finding. Thus, our study evidenced the presence of new genes and alleles involved in aluminum tolerance in maize.

3
  • MARIA EDUARDA JEUNON
  • Evaluation of cytotoxicity of extracts from the Ozoroa obovata plant in glioblastoma cell lines: in vitro and in vivo.

  • Líder : ROSY IARA MACIEL DE AZAMBUJA RIBEIRO
  • MIEMBROS DE LA BANCA :
  • ROSY IARA MACIEL DE AZAMBUJA RIBEIRO
  • FABIO VIEIRA DOS SANTOS
  • ANA GABRIELA SILVA OLIVEIRA
  • Data: 29-feb-2024


  • Resumen Espectáculo
  • xxx

4
  • LORENA SILVA FERNANDES
  • Functional analysis of SbWRKY1 transcripton fator for aluminum tolerance em Arabidopsis

  • Líder : JURANDIR VIEIRA DE MAGALHÃES
  • MIEMBROS DE LA BANCA :
  • JURANDIR VIEIRA DE MAGALHÃES
  • CLAUDIA TEIXEIRA GUIMARAES
  • JANAÍNA DE OLIVEIRA MELO
  • Fernanda Prieto Bruckner
  • Data: 14-mar-2024


  • Resumen Espectáculo
  • The development of the sorghum root system is compromised when exposed to ionic
    aluminum (Al3+) on acidic soils (pH < 5), resulting in reduced absorption of water and
    nutrients and, consequently, causing losses in crop productivity. Root release of
    organic acids, which form non-toxic complexes with Al, is the main physiological
    mechanism of Al tolerance in plants. In root aplical cells, this mechanism is mediated
    by malate and citrate transporters belonging to the aluminum-activated malate
    transporter (ALMT) and multidrug and toxic compound extrusion (MATE) families,
    respectively, whose expression is regulated by transcription factors (TFs). Most of the
    TFs involved in Al tolerance belong to the “zinc finger” and WRKY families. The TF
    AtSTOP1 (sensitive to proton rhizotoxicity 1) controls, in Arabidopsis, the expression
    of the AtALMT1 gene, which is the main Al tolerance gene in this species, as well as
    AtMATE expression. In sorghum, Al tolerance is controlled by SbMATE, which is
    transcriptionally controlled by the TFs, SbWKRY1 and SbZNF1. The objective of this
    work was to characterize the effect of the sorghum transcription factor, SbWRKY1,
    on aluminum tolerance in Arabidopsis thaliana, to elucidate the signaling pathways
    controlled by SbWRKY1. For this, Al tolerance gene expression analyses for the
    main Al tolerance genes in Arabidopsis were carried out. Transgenic plants in which
    the expression of AtSTOP1 and AtMATE was perturbed by the insertion of T-DNA, as
    well as the double mutant AtMATE/AtALMT1 were genetically transformed with the
    SbWRKY1 gene. As expected, perturbation of Al tolerance genes led to reduced Al
    tolerance in the mutant stocks. In general, complementation of the tolerance
    phenotype f(measured via relative growth of the seminal root in Al3+-containing
    solution) was observed in events transformed with SbWKRY1, in all mutant genetic
    backgrounds. Our gene expression and tolerance results led us to conclude that
    SbWRKY1 increases aluminum tolerance in Arabidopsis, acting in an alternative
    pathway to that controlled by the AtMATE or AtALMT1 genes. This alternative
    pathway, still unknown, may be partially coincident with pathways regulated by
    AtSTOP1, since SbWRKY1 complements the aluminum sensitivity phenotype in the
    AtSTOP1-KO mutant. Given the existence of alternative targets of AtSTOP1, this
    10 work indicates that there is potential for increased Al tolerance by combining distinct
    Al tolerance mechanisms.

Tesis
1
  • ISABELA FIGUEIREDO DE OLIVEIRA
  • Contribution of mycorrhizal and bacterial colonization to the efficiency of phosphorus acquisition, productivity and rhizosphere microbiome of sorghum plants.

  • Líder : SYLVIA MORAIS DE SOUSA TINOCO
  • MIEMBROS DE LA BANCA :
  • SYLVIA MORAIS DE SOUSA TINOCO
  • IVANILDO EVODIO MARRIEL
  • ARTHUR PRUDÊNCIO DE ARAÚJO PEREIRA
  • CHRISTIANE ABREU DE OLIVEIRA PAIVA
  • MARIA LUCIA FERREIRA SIMEONE
  • PAULO IVAN FERNANDES JUNIOR
  • Data: 08-abr-2024


  • Resumen Espectáculo
  • xxx

2023
Disertaciones
1
  • Amalia Amorim Silva Ribeiro Benedito
  • PRODUCTION, PURIFICATION OF RECOMBINANT HUMAN ACTIVIN AND ITS EFFECTS ON SUPEROVULATION IN FEMALE MICE.

  • Líder : JORGE LUIZ PESQUERO
  • MIEMBROS DE LA BANCA :
  • JORGE LUIZ PESQUERO
  • PAULO HENRIQUE DE ALMEIDA CAMPOS JUNIOR
  • IVAN CARLOS DOS SANTOS
  • MARIA APARECIDA GOMES
  • Data: 07-jul-2023


  • Resumen Espectáculo
  • XXX

2
  • LOURENÇO VÍTOR SILVA FERREIRA
  • GENOMIC ANALYSIS OF SELECTED BACILLUS STRAINS UNDER OSMOTIC STRESS AIMING TO IDENTIFY GENES RELATED TO PLANT GROWTH PROMOTION.

  • Líder : SYLVIA MORAIS DE SOUSA TINOCO
  • MIEMBROS DE LA BANCA :
  • SYLVIA MORAIS DE SOUSA TINOCO
  • ANDRE LUIZ MARTINEZ DE OLIVEIRA
  • ELIANE APARECIDA GOMES
  • ISABEL RODRIGUES GERHARDT
  • UBIRACI GOMES DE PAULA LANA
  • Data: 11-dic-2023


  • Resumen Espectáculo
  • Drought is a multidimensional stress that affects plants at various levels of organization. The use of plant growth-promoting bacteria (PGPB) presents itself as a very promising alternative to increase agricultural production under conditions of abiotic stress, mainly due to its low cost and reduced environmental impact. Among PGPBs, the Bacillus genus has stood out as potential plant growth promoters due to its multifunctional characteristics. This work aimed to characterize the genome of five Bacillus strains from the Embrapa Maize and Sorghum Multifunctional Microorganism Collection, isolated from the Caatinga biome, to predict plant growth-promoting characteristics, mainly related to tolerance to drought stress. The bacterial genomes were sequenced on the Illumina HiSeq 4000 platform at the Beijing Genomics Institute (BGI), using the 150 paired-end strategy. The sequenced reads were assembled using the SPAdes software and verified by the BUSCO and QUAST software, with sequence alignment performed by the MAUVE software. The genomes were annotated using PROKKA, RAST, eggNOG, BlastKOALA and antiSMASH software. Phylogenetic analysis was performed with the entire genome sequenced, using the TYGS software, through classification and identification based on complete genomes. The total size of the genomes varied between 3.9 and 4.2 million base pairs (bp), assembled between 27 and 42 contigs. GC content ranged from 43.5% to 46.5%, with an N50 ranging from 4,7×105 to 2,1×106 bp, which presents the size of the smallest contig corresponding to half of the total genome size, indicating high quality of genome assembly. The number of coding sequences obtained from the annotations ranged from 3,9×103 to 4,5×103. The phylogenetic tree based on the complete genome revealed that strains 6E9 and 5D5 clustered with Bacillus velezensis species, while strains 2E7, 1A11 and 1H10 clustered with Bacillus subtilis species. It was possible to identify genes involved in responses to heat shock, osmoregulation, endospore formation, exopolysaccharides and siderophores, biofilm production, production of phytohormones, nitrogen fixation and other responses to osmotic and oxidative stress. There are few differences in the presence of genes between the strains, however, B. velezensis and B. subtilis have some differences, such as in the choline synthesis operon. The studied strains are promising candidates for use as microbial inoculants with the potential to mitigate the harmful effects of drought stress, regulate morphophysiological characteristics, and promote plant growth.

3
  • SAMUEL FELIPE AZEVEDO GALVÃO
  • Genomic analysis and monitoring of plant growth-promoting bacteria.

  • Líder : SYLVIA MORAIS DE SOUSA TINOCO
  • MIEMBROS DE LA BANCA :
  • CHRISTIANE ABREU DE OLIVEIRA PAIVA
  • DIEGO MAURICIO RIANO PACHON
  • SYLVIA MORAIS DE SOUSA TINOCO
  • UBIRACI GOMES DE PAULA LANA
  • VERA LUCIA DOS SANTOS
  • Data: 19-dic-2023


  • Resumen Espectáculo
  • XXX

Tesis
1
  • LILIAN DE SOUZA MARQUES
  • Soil quality bioindicators in Crop-Livestock-Forest Integration Systems (ILPF) with different levels of technological investment in the central region of Minas Gerais.

  • Líder : IVANILDO EVODIO MARRIEL
  • MIEMBROS DE LA BANCA :
  • IGOR RODRIGUES DE ASSIS
  • ANIBAL DA FONSECA SANTIAGO
  • ELIANE APARECIDA GOMES
  • IVANILDO EVODIO MARRIEL
  • SIMONE MARTINS MENDES
  • Data: 20-sep-2023


  • Resumen Espectáculo
  • xxx

2
  • VITÓRIA PALHARES RIBEIRO
  • Genomic and phenotypic analysis of Azospirillum sp. and its agronomic potential in coinoculation with Bacillus sp. in maize

  • Líder : IVANILDO EVODIO MARRIEL
  • MIEMBROS DE LA BANCA :
  • IVANILDO EVODIO MARRIEL
  • SYLVIA MORAIS DE SOUSA TINOCO
  • CHRISTIANE ABREU DE OLIVEIRA PAIVA
  • UBIRACI GOMES DE PAULA LANA
  • VERA LUCIA DOS SANTOS
  • MARIANA LOURENÇO CAMPOLINO
  • ELIANE APARECIDA GOMES
  • Data: 05-dic-2023


  • Resumen Espectáculo
  • Using bacterial inoculants as plant growth promoters has been considered a practical and sustainable approach compared to chemical fertilizers and agricultural pesticides. This work aimed to characterize the strains CMS2142 of Azospirillum brasilense and CMS1626 of A. argentinense and evaluate their potential to promote plant growth. The two strains were used in single inoculations and co-inoculated with the commercial product BiomaPhos® formulated with Bacillus subtilis CNPMS B2084 e B. megaterium CNPMS B119. Field experiments were conducted on maize crops in Brazil's Southeast and Central-West regions in two agricultural seasons (2019/2020 and 2020/2021). The experiments under field conditions were conducted with seven treatments being three single treatments with the individual strains of Azospirillum sp. and BIOMAPHOS, three co-inoculations, and negative control (not inoculated). The experimental design was in randomized blocks and three replicates. The parameters evaluated were grain productivity, nitrogen (N), phosphorus (P), and potassium (K) content in the grains, enzymatic activities of acid and alkaline phosphatase, β-glycosidase, and arylsulfatase in rhizospheric soil. The results indicated significant differences for all variables depending on the year and region. Maize plants inoculated with A. argentinense CMS1626 showed high grain productivity in 100% of the cases analyzed. In general, this strain responded better when co-inoculated with BiomaPhos®. A significant positive correlation was found between productivity and nutrient content. Likewise, for biological attributes, significant differences were detected between treatments for the enzymes tested. Furthermore, the genomes of both strains of Azospirillum were sequenced, showing that the total size of the genomes varied between 6.9 and 7.7 million base pairs (bp). The GC content was 68.43% for A. argentinense (CMS1626) and 68.59%  for A. brasilense (CMS2142). Partial genome analysis showed the presence of genes involved in cyst formation, chemotaxis, motility, competition in the rhizosphere, and plant growth promotion mechanisms. Both strains fixed nitrogen and produced biofilm and siderophores in vitro. The strain CMS1626 produced more phosphatases and AIA and solubilized more phosphate, while strain CMS2142 was characterized as salinity tolerant. The growth-promoting characteristics evaluated in vitro were corroborated by genomic analysis showing genes involved in different metabolic pathways. It can be concluded that the potential of single inoculation of Azospirillum and co-inoculation with Bacillus in maize crops and the genomic characterization of growth promotion mechanisms highlighted the understanding of plant-bacteria interaction and demonstrated the potential of both strains in formulations of new inoculants for more sustainable agricultural production.

3
  • JESSICA NAIARA LARA
  • IN VITRO STUDY OF THE RELATIONSHIP BETWEEN HYPOPOTASSEMIA, HYPOMAGNESEMIA AND SPREADING DEPRESSION: A POSSIBLE EXPLANATION FOR THE MECHANISM OF MIGRAINE AURA

  • Líder : ANTONIO CARLOS GUIMARAES DE ALMEIDA
  • MIEMBROS DE LA BANCA :
  • ANTONIO CARLOS GUIMARAES DE ALMEIDA
  • LUIZ EDUARDO CANTON SANTOS
  • ANTONIO MARCIO RODRIGUES
  • CARLA ALESSANDRA SCORZA BAHI
  • RUBEM CARLOS ARAUJO GUEDES
  • Data: 15-dic-2023


  • Resumen Espectáculo
  • xxxx

2022
Disertaciones
1
  • ÉRICA BASTOS CORRÊA GONÇALVES
  • Associative mapping to identify candidate genes related to a lower incidence of fumonisin in corn kernels.

  • Líder : MARIA MARTA PASTINA
  • MIEMBROS DE LA BANCA :
  • CLAUDIA TEIXEIRA GUIMARAES
  • JANAÍNA DE OLIVEIRA MELO
  • KAIO OLIMPIO DAS GRAÇAS DIAS
  • MARIA MARTA PASTINA
  • Data: 24-ene-2022


  • Resumen Espectáculo
  • Corn production faces a number of diseases that cause contamination and are hazardous to health,
    decreasing yield and profit. Thus, plant breeding is one of the most effective and environmentally
    safe methods to control fungal infections and increase resistance to the incidence of fumonisin.
    However, conventional breeding can be hampered by the complex genetic architecture of resistance
    to fumonisin and marker-assisted selection are proposed as efficient alternatives. The aim of the
    study is to identify the genomic regions and candidate genes associated with the smallest increase
    in fumonisins in maize-simple hybrid grains. A panel of 335 inbred lines of maize was evaluated
    in the intermediate and elite trials of the maize breeding program of Embrapa Milho e Sorgo, in
    three consecutive years. The determination of fumonisin was carried out at Embrapa Milho e Sorgo.
    The strains were genotyped by sequencing (GBS), generating 374667 high quality polymorphic
    SNPs. Thirty-two possible SNPs were found associated with resistance to increased fumonisin in
    corn. Based on the SNPs identified in the associative analyzes, candidate genes with a possible
    effect on the result of fumonisins in corn kernels, the genomic regions and the genes will be
    searched. The results obtained will be compared with studies already carried out by the Embrapa
    Milho e Sorgo group.

2
  • NATÁLIA GOMES XAVIER
  • Phenotypic diversity and associative mapping for bioactive compounds in father-in-law for human consumption.

  • Líder : MARIA MARTA PASTINA
  • MIEMBROS DE LA BANCA :
  • ANTONIO AUGUSTO FRANCO GARCIA
  • JANAÍNA DE OLIVEIRA MELO
  • JURANDIR VIEIRA DE MAGALHÃES
  • KARINE DA COSTA BERNARDINO
  • MARIA MARTA PASTINA
  • Data: 27-ene-2022


  • Resumen Espectáculo
  • In recent years, there has been a growing increase in interest in using sorghum for human consumption, mainly due to the demand for healthier, gluten-free foods with greater nutritional value and a source of bioactive compounds. Bioactive compounds are secondary metabolites that act on living organisms and provide many health benefits, as they have antioxidant, immunomodulatory, anti-osteoporotic, anti-hypertensive, anti-inflammatory, anti-microbial, anti-diabetic and anti-cancer properties, and are considered effective in reducing cardiovascular complications. However, the accessibility, availability and chemical characteristics of bioactive compounds have wide variability between different sorghum genotypes, which results in different behaviors in the food matrix and in the interaction with other food ingredients. As a result, genetic improvement programs have invested efforts in the identification, characterization and quantification of compounds, which is essential for a better understanding of their synthesis pathways and their genetic regulation. In this context, the objectives of the present work were: i) to verify the phenotypic diversity in a panel of sorghum lines evaluated for the composition of bioactive compounds in the grains; ii) to identify, from associative mapping analyzes, genomic regions associated with the concentration of tannins, total phenolics, luteolinidine chloride, apigenenidine chloride and resistant starch in sorghum grains; iii) to develop gene-specific molecular markers for the Tannin 1 gene, which regulates the tannin biosynthesis in sorghum. Thus, the results of the present work can contribute to the discovery of new genes related to the biosynthesis pathway of bioactive compounds in sorghum, in addition to enabling a better understanding of the phenotypic and genetic diversity for bioactive compounds in sorghum. Additionally, the development of a molecular marker for the Tannin 1 gene that can assist the process of genotype selection in Embrapa's sorghum breeding program.

     

     

     

3
  • FERNANDA DE CÁSSIA BATISTA
  • SELECTION OF IRON PHOSPHATE SOLUBILIZING BACTERIA TO INCREASE PHOSPHORUS USE IN CORN

  • Líder : IVANILDO EVODIO MARRIEL
  • MIEMBROS DE LA BANCA :
  • CHRISTIANE ABREU DE OLIVEIRA PAIVA
  • DANIEL BINI
  • IVANILDO EVODIO MARRIEL
  • MARCIA REGINA DA COSTA
  • SWIANY SILVEIRA LIMA
  • Data: 10-jun-2022


  • Resumen Espectáculo
  • XXX

4
  • JEAN CRISTO TEIXEIRA CIARALLO
  • Efeito da proteína recombinantes Kint3-4 sore as atividades epileptiformes não sinápticas em hipocampo de rato

  • Líder : ANTONIO CARLOS GUIMARAES DE ALMEIDA
  • MIEMBROS DE LA BANCA :
  • ANTONIO CARLOS GUIMARAES DE ALMEIDA
  • ANTONIO MARCIO RODRIGUES
  • CARLA ALESSANDRA SCORZA BAHI
  • Data: 29-jun-2022


  • Resumen Espectáculo
  • ------------

5
  • BRUNA CÁSSIA RODRIGUES DE LIMA
  • Influence of manganese ions on non-synaptic epileptiform activities..

  • Líder : ANTONIO CARLOS GUIMARAES DE ALMEIDA
  • MIEMBROS DE LA BANCA :
  • ANTONIO CARLOS GUIMARAES DE ALMEIDA
  • ANTONIO MARCIO RODRIGUES
  • FERNANDO HENRIQUE MAGALHAES
  • Data: 26-ago-2022


  • Resumen Espectáculo
  • xxxx

6
  • Bernardo Camara do Nascimento
  • THE ADMINISTRATION OF NAPROXEN LEADS TO ADVERSE EFFECTS ON THE OVARIAN MORPHOPHYSIOLOGY OF MICE

  • Líder : PAULO HENRIQUE DE ALMEIDA CAMPOS JUNIOR
  • MIEMBROS DE LA BANCA :
  • PAULO HENRIQUE DE ALMEIDA CAMPOS JUNIOR
  • MILLER PEREIRA PALHÃO
  • LUÍZ GUSTAVO BRUNO SIQUEIRA
  • Data: 01-nov-2022


  • Resumen Espectáculo
  • xxx

7
  • Lucas Rolim Medaglia
  • DEVELOPMENT AND APPLICATION OF MINIDOCKING AGAINST THE MAIN PROTEASES AND SIMILAR TO PAIN OF SARS-COV-2

  • Líder : ALEX GUTTERRES TARANTO
  • MIEMBROS DE LA BANCA :
  • ALEX GUTTERRES TARANTO
  • LUIS FERNANDO SOARES
  • MARCELO SIQUEIRA VALLE
  • MOACYR COMAR JUNIOR
  • Data: 19-dic-2022


  • Resumen Espectáculo
  • xxx

Tesis
1
  • Izabelle Gonçalves Melo
  • ENVIRONMENTAL QUALITY MONITORING OF THE SOIL UNDER THE CROP-LIVESTOCK-FORESTRY INTEGRATION SYSTEM (ILPF) IMPLEMENTED IN THE CENTRAL REGION OF MINAS GERAIS UNDER CERRADO

  • Líder : IVANILDO EVODIO MARRIEL
  • MIEMBROS DE LA BANCA :
  • IVANILDO EVODIO MARRIEL
  • MARCIA REGINA DA COSTA
  • MARIA CELUTA MACHADO VIANA
  • MIGUEL MARQUES GONTIJO NETO
  • SWIANY SILVEIRA LIMA
  • Data: 04-jul-2022


  • Resumen Espectáculo
  • The world population has grown in unprecedented ways and along with that, the
    demand to produce healthy and cheap food in a sustainable and profitable way. The
    Crop-Livestock-Forest Integration System (ICLF) system has been shown to be a
    sustainable alternative for land use and conservation of natural resources. This
    cultivation strategy aggregates in a single area different production systems, such as
    wood, grains, meat and milk, allowing the maximum use of the area. Knowledge of
    the diversity of soil microbial ecology and its activity under ICLF becomes of
    fundamental importance to assess the stability of these systems. This study aimed to
    evaluate changes in soil quality in Cerrado under ICLF involving different structural
    arrangements of eucalyptus and pasture, in two experimental areas in the central
    region of Minas Gerais (EMBRAPA Maize and Sorgum, and Santa Rita Farm –
    EPAMIG). The levels of TOC, LC and RBS were monitored, in addition to the
    activities of arginase, urease, acid and alkaline phosphatase, β-glucosidase and
    Arylsulfatase, in soil samples collected at EPAMIG in three ILPF arrangements (rows
    of 9 m with single and double rows of eucalyptus, and 20 m with double rows of
    eucalyptus) planted in 2008 (1 m away from the eucalyptus trunk and in the center of
    the row), and using pasture areas in full sun, eucalyptus GG100 (3x3) and native
    Cerrado as a control. The experimental design was randomized blocks in split plots,
    with three replications. In the EMBRAPA experimental area, soil samples were also
    collected in three ICLF arrangements (rows of E. urophylla cv. GG100 implanted in

    2009, 2011 and 2013). The experimental design was randomized blocks in sub-
    divided plots, with three replications. In each repetition, samples were taken,

    composed of five subsamples, at four depths (0-10, 10-30, 30-60 and 60-100 cm and
    0-10, 10-20, 20-40 and 40-60 cm for EPAMIG and EMBRAPA, respectively). As a
    result, there was a significant influence (p<0.05) of the sampling season factor on all
    parameters evaluated, and, in general, the highest microbial activity was observed in
    March/2019, the period with the highest precipitation rate. The mean values for CL
    increased as a function of the presence of water in the system; RBS with increased
    values as a function of the time of implementation of the ICLF system; β-Glucosidase
    enzyme activity corroborated the results found for LC at the same time of evaluation.
    It was concluded that the use of the bioindicators used allowed to detect changes in

    X

    the biological quality of the soil of the Cerrado biome under different ILPF
    arrangements, demonstrating that there is an increase in the quality of the soil over
    the time of implementation of the system.

2
  • MAYRA CONSUELO AARAO
  • Expression of non-synaptic mechanisms and epileptform activities in gerbils Meriones unguiculatus

  • Líder : ANTONIO CARLOS GUIMARAES DE ALMEIDA
  • MIEMBROS DE LA BANCA :
  • ANTONIO CARLOS GUIMARAES DE ALMEIDA
  • ANTONIO MARCIO RODRIGUES
  • CARLA ALESSANDRA SCORZA BAHI
  • FULVIO ALEXANDRE SCORZA
  • SERGIO GOMES DA SILVA
  • Data: 14-jul-2022


  • Resumen Espectáculo
  • ---------

3
  • DANIELLE STORINO DE FREITAS
  • MATERNAL FOOD SUPPLEMENTATION WITH CONJUGATED LINOLEIC ACID (CLA) ON OVARIAN MORPHOPHYSIOLOGY AND FERTILITY OF PROGENY MICE.

  • Líder : PAULO HENRIQUE DE ALMEIDA CAMPOS JUNIOR
  • MIEMBROS DE LA BANCA :
  • PAULO HENRIQUE DE ALMEIDA CAMPOS JUNIOR
  • ROSY IARA MACIEL DE AZAMBUJA RIBEIRO
  • RIBRIO IVAN TAVARES PEREIRA BATISTA
  • ERIKA CRISTINA JORGE
  • MILLER PEREIRA PALHÃO
  • Data: 10-oct-2022


  • Resumen Espectáculo
  • xxx

2021
Disertaciones
1
  • MONICA PEREIRA CAMPANHA VIEGAS
  • EVALUATION OF NON-SYNAPTIC MECHANISMS INVOLVED IN PARKINSON'S DISEASE

  • Líder : ANTONIO CARLOS GUIMARAES DE ALMEIDA
  • MIEMBROS DE LA BANCA :
  • FULVIO ALEXANDRE SCORZA
  • LUIZ EDUARDO CANTON SANTOS
  • ANTONIO CARLOS GUIMARAES DE ALMEIDA
  • CARLA ALESSANDRA SCORZA BAHI
  • Data: 29-ene-2021


  • Resumen Espectáculo
  • Introduction:Parkinson's disease (PD) is characterized by dopaminergic neuronal loss of the substantia nigra (SN), with generalized accumulation of intracellular alpha-synuclein. Some symptoms of the disease may be directly linked to neuronal loss, while others appear to be caused by aberrant activities and cellular changesin the base nucleus circuit, which can increase the oscillatory trigger and synchronization ofsurviving neurons. There are non-synaptic mechanisms of GABA neurotransmission modulation, directly related to the activity of the base nuclei, which may be involved in this process, leading to inhibitory and excitatory imbalances in the neuronal circuit,with the generation of asynchronous activities. Objectives:analysis of changes in the activity of non-synaptic mechanisms such as Na + / K + ATPase pump, glial cells, co-transporters, tyrosine hydroxylase and Cx36 that can modify how GABA reform and leadto a loss of balance, excitation / inhibition , assisting in the initiation and perpetuation of the PD neurodegenerative process. Methodology:16 male Wistar rats, 3 months old, randomly divided into two groups, being DP24 (treated withunilateral stereotaxic injection of 24μg of 6-OHDA in the striatum) and Control (with replacement of 6-OHDA by saline 0.9%). After 15 days, the brains were sectioned and stained by immunofluorescence against Tyrosine hydroxylase, Cx36, KCC1, NKCC2, Na + / K + -ATPase pump and GFAP. Then they were found by confocal microscopy. Results and Discussion:The model of 24ug injection of 6-OHDAwas effective in promoting experimental parkinsonism in rats, evidenced by the death of TH + neurons. Cell death was accompanied by gliosis, which was evidenced by increased GFAP expression. The glial hyperactivity associated with the increase in lipofuscin is evidence of the neuroinflammatory process, which seems to be related to the synchronizing imbalances already observed in PD, which generate disturbance in the structure of the neuronal network. In human tissue, the death of dopaminergic neurons leads to remodeling of connexins 36. In the present study, the results of the unilateral analysis of variance confirmed the significant difference between the areas evaluated. Even the contralateral region underwent positive modulation in Cx36 expression due to the injection of 6-OHDA. This restructuring would be the possible place for the propagation of neurotoxic substances and alpha-synuclein, with disturbance of the excitatory / inhibitory balance in the synapses. The reorganization of the neuronal circuitry triggered by the neuroinflammatory process can lead to an imbalance in neurotransmission with a decrease in the threshold for crises and a consequent increase in the synchronizing capacity. There was no detection of changes in the expression of the KCC1 and KCC2 co-transporters and Na + / K + -ATPase pump, which seems to be related to the time used for their analysis after stereotaxic surgery(15 days). Conclusion: The present protocol demonstrated significant changes in the variation of TH, GFAP and CX36, which may be related to toxic neuroinflammatory activity and changes in the synchronization mechanisms. Our results raise new therapeutic possibilities, with the premise of using GJ blockers directly over nigrostriatal pathways, in order to reduce / control the aberrant synchronism and, thus, control the symptoms of PD.

2
  • EMILY AUGUSTA ANDRADE PÁDUA
  • ELECTROPHYSIOLOGICAL EVALUATION OF NON-SYNAPTIC CONNECTIONS IN A MOUNTAIN HYPOCAMPLE THAT EXPRESSES RAT TONE.

  • Líder : JORGE LUIZ PESQUERO
  • MIEMBROS DE LA BANCA :
  • ANTONIO CARLOS GUIMARAES DE ALMEIDA
  • IVAN CARLOS DOS SANTOS
  • JORGE LUIZ PESQUERO
  • LUIZ EDUARDO CANTON SANTOS
  • MARIA APARECIDA GOMES
  • Data: 22-abr-2021


  • Resumen Espectáculo
  • Patients with temporal lobe epilepsy have drug refractoriness, which makes it
    necessary to investigate new pathways and therapeutic approaches, such as the
    involvement of non-synaptic factors and the participation of the angiotensin tonin
    system in epileptic seizures. The enzyme tonin allows the direct conversion of
    angiotensinogen to angiotensin II, the latter, at the brain level, favors the occurrence
    of crises. Thus, the work aimed to characterize the non-synaptic epileptiform activities
    in the transgenic mouse that expresses TGM rat tonin (rTon) and in its control C57bl6.
    Thus, after identifying the presence of the transgene for tonin by means of genotyping,
    electrophysiological recording of the hippocampal slices of the mice was performed
    using the protocol for inducing non-synaptic activities. The electrophysiological
    parameters showed that the TGM (rTon) animals showed latency time and interval
    between minor events and longer event duration when compared to controls. The
    maximum amplitude of the population spykes was greater in the TGM (rTon) and the
    average of the populations spykes smaller in relation to the control. The results
    obtained suggest that TGM (rTon) animals are more susceptible to non-synaptic in
    vitro epileptiform crises. One of the justifications would be the participation of the tonin-
    angiotensin system by the release of Angiotensin (Ang II) by inhibiting potassium
    channels activated by voltage type IA by 50% which leads to increased excitabilitywhich could justify the increase in the duration of the event. Another hypothesis would
    be the action of connexins promoting positive regulation for seizures, in special
    connexin 43.

3
  • THAISLANE SABRINA DE SOUZA OLIVEIRA
  • Soil microbiological attributes as sustainability indicators of grain production systems in the central cerrado of Minas Gerais

  • Líder : IVANILDO EVODIO MARRIEL
  • MIEMBROS DE LA BANCA :
  • IVANILDO EVODIO MARRIEL
  • FREDERICK MENDES AGUIAR
  • DENISE PACHECO DOS REIS
  • ÁLVARO VILELA DE RESENDE
  • Data: 03-ago-2021


  • Resumen Espectáculo
  • xxx

4
  • JOSÉ IBRAHIM EL-CORAB NETO
  • Selection and characterization of endophytic yeasts of sweet sorghum fermenting xylose and producing hydrolytic enzymes with potential use in the production of xylitol and ethanol

  • Líder : IVANILDO EVODIO MARRIEL
  • MIEMBROS DE LA BANCA :
  • IVANILDO EVODIO MARRIEL
  • JACQUELINE APARECIDA TAKAHASHI
  • MARIA LUCIA FERREIRA SIMEONE
  • UBIRACI GOMES DE PAULA LANA
  • Data: 30-ago-2021


  • Resumen Espectáculo
  • The demand for using lignocellulosic material is constantly increasing. Some microorganisms are able
    to convert the sugars present in this material transforming them into ethanol and xylitol. Hydrolytic enzymes play an important role in several processes of biotechnological interests. Among these processes, the use of biomass to produce biofuels and xylitol, as an alternative to non-renewable sources, has aroused great interest due to climate change. The objective of this work was to evaluate and select yeasts that produce the hydrolytic enzymes amylase, cellulase and xylanase, with potential for ethanol and xylitol production. The selection of yeast strains was determined by growth in
    culture media with differentcarbonsources(solublestarch,carboxymethylcellulose - CMC and xylan), in triplicate. The evaluation of theenzymatic activity was carried out through the enzymatic index (IEA), and throughthe quantification of enzymes by fermentation in liquid medium for the enzymesamylase, cellulase (CMCase and Fpase) and xylanase. After quantification inliquid medium, the best strains with enzyme production results were selected tocarry out the fermentation in medium containing xylose as the only carbon source.Lines3.22Meyerozymaguilliermondiiand3.26Torulasporadelbrueckiimanaged to produce the three analyzed enzymes. In total, seven strains wereselected with the capacity to ferment glucose to ethanol to ferment the mediumcontaining xylose, among them: 3.6, 4.8, 3.22, 3.26, 4.16, 4.10 and 4.5. Sixstrains managed to assimilate xylose producing xylitol. The strain that producedthe highest amount of xylitol was 3.6 Wickerhamomyces anomalus, with aproduction of 7.666 gL-1, with a xylose to xylitol conversion factor of Yp/s=0.481 (gg-1) and productivity Qp=10.221 (gL- 1.h-1). A new strain capable of fermenting xylose into xylitol was identified, being the species Torulaspora delbrueckii.

Tesis
1
  • JOÃO PAULO SACRAMENTO
  • ENERGY METABOLISM IN LACTATION COWS: PARTICIPATION, METHANE PRODUCTION AND ENERGY REQUIREMENTS.

  • Líder : LUIZ GUSTAVO RIBEIRO PEREIRA
  • MIEMBROS DE LA BANCA :
  • CAMILA FLAVIA DE ASSIS LAGE
  • FERNANDA SAMARINI MACHADO
  • JOÃO PAULO PACHECO RODRIGUES
  • LUIZ GUSTAVO RIBEIRO PEREIRA
  • THIERRY RIBEIRO TOMICH
  • Data: 10-jun-2021


  • Resumen Espectáculo
  • 18The objective was to evaluate the requirement of maintenance and net and metabolizable
    19energy of crossbred Holstein x Gyr cows throughout a whole lactation. Likewise,
    20energetic efficiency parameters as the efficiency of utilization of the ME intake for milk
    21production (kL), tissue gain (kG), and utilizing body stores for milk production (kT).
    22Twenty-nine Holstein x Gyr crossbred cows with an average initial weight of 563.40 ±
    2340.08 and 2.45 ± 0.09 years were used throughout a whole lactation. The animals were
    24first-calving, already in an adult weight, and during the study, the cows were non-
    25pregnant to decrease any confounding factor. Cows were fed immediately after milking
    26twice a day in amounts to allow ad libitum intake (allowing for 5 to 10% orts). The intake
    27and milk production were determined daily. Apparent digestibility assay followed by a
    28calorimeter measurements was performed at the beginning and the end of each lactation
    29period, approximately 45 to 45 days in milk (DIM). Subsequently completed the lactation,
    30the dry cows were fed the diet used at the late lactation stage limited at 1.1% of BW of
    31DMI to prevent change in body weight (maintenance level). After 21 days of diet
    32adaptation, an apparent digestibility assay followed by calorimeter measurements was
    33performed. Fasting measurement was performed later than the maintenance period where
    34the cows were housed individually inside the chambers for 72 hours without access to
    35food. Data were analyzed using the MIXED procedure of SAS (SAS version 9.4; SAS
    36Inst. Inc., Cary, NC) according to a completely randomized design. Crossbred Holstein ×
    BW0.75/BW0.75.
    37Gyr dairy cow showed a MEM of 0.588 MJ/and NEM of 0.395 MJThe
    38efficiency of dietary ME utilizing for producing milk, gain, and the efficiency of utilizing
    body stores39for milk production were 0.67, 0.77, and 0.81, respectively. Crossbred/BW0.75
    40Holstein × Gyr dry and non-pregnant cows showed a MEM of 0.434 MJ
    and NEM BW0.75 BW0.75
    41of 0.351 MJ/for maintenance and a MEM of 0.396 MJ/
    and NEM of 0.345BW0.75
    42MJ/for fasting metabolism. The efficiency of utilizing dietary ME for maintenance
    43(kM) was 0.80. The results confirmed that F1 crossbreed Holstein × Gyr dairy cows have
    44differences in the requirement of energy throughout the lactation stages, however,
    45comparing with data available in the literature, there are no differences between crossbred
    animals raised under tropical and purebred cows.

2
  • MICHELLE OLIVEIRA CAMPAGNANI
  • Endophytic inoculation of new entomopathogenic fungi as a
    means of controlling insect pests
  • Líder : WELLINGTON GARCIA DE CAMPOS
  • MIEMBROS DE LA BANCA :
  • ALEXANDER MACHADO AUAD
  • JOSÉ EDUARDO MARCONDES DE ALMEIDA
  • LUIZ HENRIQUE ROSA
  • ROGERIO MARTINS MAURICIO
  • WELLINGTON GARCIA DE CAMPOS
  • Data: 14-sep-2021


  • Resumen Espectáculo
  • Entomopathogenic fungi are microorganisms that are insect pathogens and plant
    symbionts, with diverse ecological interactions. The fungi UFMG 11444 and UFMG
    11443 were isolated from Mahanarva spectabilis in a silvipastoral system and showed
    potential to be used in biological pest control. The conidia of these fungi were produced
    1012 in a 1:1 mixture of corn and rice flour, and batches with 1 x
    conidia/g were obtained. The fungi UFMG 11444 and UFMG 11443 grew more than 80% when
    exposed to 40°C and below 35º C germinated more than 92% of the conidia. With less
    than 45 minutes of exposure to solar radiation, both fungi UFMG 11443 and 11444
    germinated more than 80%. These, inoculated via seed treatment, foliar spraying and
    in the roots by watering young plants with the fungal suspensions, colonized more than
    60% of the samples inducing endophytia, promoted biomass gain and showed no signs
    of phytopathogenicity in plants such as Glycine max, Oryza sativa, Zea mays, Triticum
    aestivum, Phaseolus vulgaris. In Urochloa brizantha under greenhouse conditions,
    testing the fungi UFMG 11444 and UFMG 11443, the inoculation techniques via seed
    10th treatment or via foliar were significantly different between them. From the
    day after exposure, in plants inoculated via the foliar route, 100% of M. spectabilis were infected.
    In the endophytic inoculation via seeds the fungi caused epizootics of more than 88%
    10th after the day. The fungi did not cause phytopathogenicity, colonized the plants and
    no significant differences in biomass gain were observed. In Brassica oleracea, aiming
    the control of Plutella xylostella and Brevicoryne brassicae in inoculation via seed, leaf
    and root, besides the fungi UFMG 11444 and UFMG 11443 a commercial strain of
    Metarhizium anisopliae was used to serve as infection parameter. It was reported
    100% efficient in controlling Brevicoryne brassicae after 15 days of exposure to the
    plants. In the tests with Plutella xylostella, inoculation via foliar spray was not used. In
    10th the inoculations via seed and root 100% of the insects died, starting from the
    day of exposure to the plants. More than 60% of B. oleracea plants were colonized by the
    fungi UFMG 11444 and UFMG 11443 and showed biomass gain and none showed
    phytopathogenic sym toms. In field tests in U. brizantha pastures aiming to control M.
    ha-1, ha -1 ha-1 spectabilis, doses of 0,5 kg 1,0 kg and 2,0 kg of UFMG 11443 and 11444 ha
    -1 were sprayed. The dose of 0,5 kg
    of both fungi was not significantly different from
    ha-1, the other doses of 1,0 and 2,0 kg
    standing out from the others for having a smaller
    volume and possibly leading to a lower cost to the producer. No significant differences
    were observed between the fungi at 6 and 10 days after inoculation in the field,
    demonstrating that both have the potential to control the population of M. spectabilis.
    It was concluded that the use of these fungi in the endophytic pathway with plants as
    vectors, has potential to be candidates for bioproducts for biological control of pests

3
  • BÁRBARA RODRIGUES NASCIMENTO
  • Temporal evaluation of the effects of vitrification with subsequent ectopic
    autologous transplantation on murine ovarian morphophysiology
  • Líder : PAULO HENRIQUE DE ALMEIDA CAMPOS JUNIOR
  • MIEMBROS DE LA BANCA :
  • ANA PAULA MADUREIRA
  • FERNANDA RADICCHI CAMPOS LOBATO DE ALMEIDA
  • JOAO HENRIQUE MOREIRA VIANA
  • LUÍZ GUSTAVO BRUNO SIQUEIRA
  • PAULO HENRIQUE DE ALMEIDA CAMPOS JUNIOR
  • Data: 21-sep-2021


  • Resumen Espectáculo
  • The vitrification of ovarian tissue followed by transplantation is a promising alternative for the
    conservation of genetic material from endangered species, wild, domestic or of high
    zootechnical value; as well as a conservation alternative before cancer treatment, allowing the
    storage of a large number of follicles and preserving fertility. The main challenge for ovarian
    graft longevity is the substantial loss of follicles during the post-transplant ischemic period.
    Therefore, the objective of our work was temporally investigate the effects of vitrification and
    autotransplantation in the murine ovarian ectopic morphophysiology. Female mice of the
    C57B1/6J lineage, adult at 6 weeks of age in the proestrus cycle phase, were randomly divided
    into control and vitrified treatments (n=79) and untransplanted (0d) or recovered 1, 12 or 23
    days were evaluated post-transplant (1, 12, 23d). The ectopic autologous transplant was
    performed to the dorsal subcutaneous region of the animal's forelimbs. After the transplant, the
    animals' estrous cycle was monitored through vaginal cytology. At the end of each treatment
    period, the ovaries were collected. Both treatments (control and vitrified) of grafts in 1d had a
    higher number of atresic follicles (p<0,0001), but an even higher rate of atresia was observed
    in the treatment vitrified in 1d (p<0,01). However, this number was attenuated by 12 and 23d
    (p<0,0001). The follicular activation rate was higher in ovaries vitrified in 1d (p<0,01) and
    significantly reduced in ovaries vitrified in 23d (p<0,05). The duration of the estrous cycle of
    both treatments was similar (p>0,05) and all females moved through all stages of the cycle. A
    reduction in oocyte and primary follicle diameter of vitrified ovaries was observed at 0d
    (p<0,01; p<0,001). In addition, at the end of 23 days, all animals in both treatments had their
    ovarian endocrine function fully reestablished and cycle duration was similar for all treatments
    (p>0,05). Blood perfusion at the transplant site was similar for both treatments (p>0,05). In 1d,
    the Bax and Casp3 genes were differentially expressed, both compared to the 0d control. Such
    treatments were also the ones that qualitatively showed more positive TUNEL cells. For both
    1d treatments, genes Acvr1, Egfr and Lhcgr were down-regulated and Fshr and Igf1r genes
    were up-regulated, both in relation to the 0d control (p<0,05). Only the Jag1 gene was
    differentially regulated between the 1d treatments compared to the 0d control. However, when comparing vitrified and control ovaries, both 1d, the genes Acvr1, Egfr, Fshr, Igf1r, Jag1 and
    Casp3 are down-regulated (p<0,05). In summary, our findings demonstrate that the period
    immediately after transplantation is critical for graft establishment due to the high rate of
    atresia. However, this loss is compensated with a higher rate of follicle activation and a decrease
    in the rate of atresia after establishing the graft at the transplant site. Furthermore, all animals
    returned their cyclicity, which demonstrates that vitrification does not affect ovarian endocrine
    function. Despite some changes in the expression of genes related to follicular survival in the
    first days post-transplantation, ovarian grafts were able to support folliculogenesis. Thus,
    vitrification with subsequent ectopic autologous transplantation seems to be a promising
    technique for the preservation of fertility.

4
  • MARIANA LOURENÇO CAMPOLINO
  • POTENTIAL OF MORPHO-PHYSIOLOGICAL RESPONSE AND RHIZOSPHERIC IN THE GROWING OF MAIZE AND SORGHUM GENOTYPES IN THE FIELD UNDER DIFFERENT LEVELS OF PHOSPHATE REACTIVITY
  • Líder : SYLVIA MORAIS DE SOUSA TINOCO
  • MIEMBROS DE LA BANCA :
  • SYLVIA MORAIS DE SOUSA TINOCO
  • CRISTIANE ALCANTARA DOS SANTOS
  • PAULO IVAN FERNANDES JUNIOR
  • MAURICIO DUTRA COSTA
  • RAFAEL SOARES CORREA DE SOUZA
  • Data: 28-oct-2021


  • Resumen Espectáculo
  • xxx

2020
Disertaciones
1
  • DAFNE MENDES SOARES
  • CATION-CHLORIDE COTRANSPORTERS AND THE GENERATION, SUPRESSION AND SUSTENTATION OF NON-SYNAPTIC EPILEPTIFORM ACTIVITY: COMPUTATIONAL SIMULATIONS


  • Líder : ANTONIO MARCIO RODRIGUES
  • MIEMBROS DE LA BANCA :
  • ANTONIO CARLOS GUIMARAES DE ALMEIDA
  • ANTONIO MARCIO RODRIGUES
  • DAMIEN THOMAS DEPANNEMAECKER
  • GISELE SILVA NOGUEIRA
  • Data: 05-mar-2020


  • Resumen Espectáculo
  • The mechanisms involved in the regulation of chloride homeostasis are intimately related to
    epileptiform activity. Therefore, studies have been investigating the effect of cation-chloride
    cotransporters on abnormal neural activity. However, the contribution of these cotransporters
    to the process of generation and sustentation of epileptiform discharges is not yet clear. This
    study aimed to investigate the effect of the blockade of cation-chloride cotransporters (KCC
    and NKCC) on the generation and sustentation of non-synaptic epileptiform activity (NSEA)
    through mathematical modeling and computational simulations. The mathematical model
    describes cellular and subcellular electrochemical mechanisms which are typical of the granular
    layer of the dentate gyrus in the rat hipoccampus, such as ion channels, the Na/K pump, the
    cotransporters KCC and NKCC, etc. The cotransporters KCC and NKCC, in particular, were
    represented according to the formalism of Michaelis-Menten, considering the reactions of their
    physiological ligands in an equilibrium state in each instant. Reproducing experimental
    procedures, the simulations show that even though the blockage of both cotransporters is not
    enough to suppress non-synaptic epileptiform discharges after they have been triggered, it is
    possible to prevent these discharges if the blockade happens before the first one. This makes it
    possible to hypothesize that drugs that target KCC and NKCC may be used to prevent epileptic
    seizures.

2
  • ANDREIA RODRIGUES CAMPOS
  • EVALUATION OF GLUCOSE METABOLISM DURING NON-SYNAPTIC EPILETIFORM ACTIVITIES - ELECTROPHYSIOLOGY.

  • Líder : ANTONIO CARLOS GUIMARAES DE ALMEIDA
  • MIEMBROS DE LA BANCA :
  • ANTONIO CARLOS GUIMARAES DE ALMEIDA
  • CARLA ALESSANDRA SCORZA BAHI
  • FULVIO ALEXANDRE SCORZA
  • LUIZ EDUARDO CANTON SANTOS
  • Data: 05-mar-2020


  • Resumen Espectáculo
  • Severe epileptic seizures are associated with a significant decrease in ATP concentration. These changes are identified as the cause of clinical sequelae. However, the whole process involved in the course of crises is not yet elucidated. Studies have been focused on the assessment of metabolic conditions necessary for the occurrence of epileptiform activities. Given the importance of glucose in maintaining ideal levels of ATP, therefore acting directly on neuronal metabolic levels, and considering that intense epileptic seizures are sustained in the absence of synaptic connections, the present study aims to investigate the effects of hypoglycemia on seizures. Experiments to induce non-synaptic epileptiform activities were performed with hippocampal slices of wistar rat. Results show suppression of non-synaptic epileptiform activities during hypoglycemic maneuvers. During these maneuvers, a series of tests sought to identify the possible mechanisms responsible for suppression. The data interpretation suggests the suppression of activities induced by hypoglycemia is due to a depolarization block.

     

3
  • JÚLIA FLÓRIO PIRES DE ANDRADE
  • The role of basil (Ocimum basilicum) inflorescences on beneficial arthropods and aphids in
    associated collard (Brassica oleracea var. acephala) plantations.

  • Líder : WELLINGTON GARCIA DE CAMPOS
  • MIEMBROS DE LA BANCA :
  • LIVIA MENDES DE CARVALHO SILVA
  • LUÍS CLÁUDIO PATERNO SILVEIRA
  • WELLINGTON GARCIA DE CAMPOS
  • Data: 17-mar-2020


  • Resumen Espectáculo
  •  

     In habitat management for conservative biological control, the vegetation diversification may
    benefit natural enemies and reduce insect pest populations. This study aimed to assess whether
    flowering basil (Ocimum basilicum L.) (Lamiaceae), used as an associated plant, increases the
    abundance of arthropods, especially predators and parasitoids, and reduces aphid (Hemiptera:
    Aphididae) abundance and infestations in collard (Brassica oleracea L. var. acephala) plantations.
    Three experimental treatments were carried out, each with two cultivation beds with ten collard
    plants and ten basil plants: (1) association between collard and flowering basil; (2) association of
    collard with basil, in which the inflorescences were removed, and (3) collard grown separately. The
    arthropods were sampled for 10 consecutive weeks, using yellow pan traps, while aphids were
    sampled by direct counting on collard leaves. The basil inflorescences increased the abundance and
    number of arthropod families in the system, while the basil without inflorescences did not improve
    arthropod abundance compared to collards grown alone. Compared to isolated collards, aphid
    abundance was lower in both combinations of collard and basil, regardless of the presence of
    inflorescences; however, inflorescences reduced the temporal frequency of aphid population peaks.
    Although flowering basil increased the abundance of aphid predators in the system, chemical
    repellents from basil may have been the main factor that reduced population aphids in collards.


4
  • EVELINE ANIELLY CRISTELLI SOARES
  • PROSPECTION OF DIAZOTHROPHIC BACTERIA AND PRODUCERS OF PHYTOHORMONES ISOLATED FROM FORAGE GRASSES GENOTYPES.

  • Líder : IVANILDO EVODIO MARRIEL
  • MIEMBROS DE LA BANCA :
  • FREDERICK MENDES AGUIAR
  • IVANILDO EVODIO MARRIEL
  • ROSANGELA MARIA SIMEÃO
  • Data: 28-ago-2020


  • Resumen Espectáculo
  • Brazil has approximately 180 million hectares of pasture, 85% of the forages being
    of the genus Urochloa Urochloa (syn. Urochloa), due to its high adaptability to
    different edaphoclimatic conditions. These plants are also an essential part of
    integrated agricultural production systems. However, about 70% of these areas are
    degraded, mainly due to inadequate management, particularly related to nitrogen
    fertilization. One of the alternatives for the supply of this nutrient in replacement /
    supplementation of nitrogen fertilizers is the use of diazotrophic bacteria, which
    perform the biological fixation of N2 (FBN). In view of this context, it was aimed; (i)
    isolate, characterize and pre-select diazotrophic and non-diazotrophic bacteria,
    aiming at inoculation of forages, and, (ii) evaluate the growth promotion in plants of
    Urochloa brizantha cv. Marandu submitted to inoculation with pre-selected bacterial
    strains. For isolation using samples obtained from rhizospheric soil, root and stem
    (endophytic) from five genotypes of forage grasses and semi-solid culture medium
    defined for Azospirillum spp. 156 bacterial isolates were obtained (53% from the
    root, 30% from rhizospheric soil and 17% stalk) which were incorporated into the
    Collection of Multifunctional Microorganisms of Embrapa Corn and Sorghum. Based
    on phenotypic characteristics and growth promotion through the production of
    indolacetic acid (AIA) and phosphate solubilization, 37 isolates were pre-selected,
    these were tested for ammonium production and activity of the enzyme ACC
    deaminase (ACCd). Among these, 30 isolates were tested for molecular
    characterization, phylogenetic affiliation and for promoting growth and nitrogen
    incorporation in plants of U. brizantha cv. Marandu. As a result, it was observed that
    75% were classified as diazotrophic, 25% as non-diazotrophic, 99.36% as AIA
    producers, 12.82% phosphorus solubilizers, 100% ammonium producers and 84%
    with ACCd activity . Analysis of the sequences of the 16S rDNA subunit of bacterial
    isolates showed high genetic diversity among isolates, with 50% identified as
    Azospirillum spp., 23% Bacillus spp., 7% Enterobacter, 3% Microbacterium, 7%
    Serratia, 3% of the genus Stenotrophomonas and 7% of the isolates were not
    identified. Regarding the growth promotion of cultivar Marandu, it was observed
    after 90 days of cultivation in a greenhouse, significant variation according to the
    strains for all tested variables: dry weight of the aerial part (PSPA); dry root weight(PSR); total green weight (PVT); total dry weight (PST); root / shoot ratio; N content
    in the aerial part. The results allowed the selection of promising strains as
    candidates for the formulation of specific inoculants for forage grasses.

Tesis
1
  • NATANAEL TAVARES DE OLIVEIRA
  • MECHANISM OF ACTION OF A BIOSTIMULANT BASED ON HUMIC SUBSTANCE ON THE DEVELOPMENT OF CORN.

  • Líder : SYLVIA MORAIS DE SOUSA TINOCO
  • MIEMBROS DE LA BANCA :
  • SYLVIA MORAIS DE SOUSA TINOCO
  • LEONARDO LUCAS CARNEVALLI DIAS
  • LUIS WILLIAN PACHECO ARGE
  • DANIEL BASILIO ZANDONADI
  • CYNTHIA MARIA BORGES DAMASCENO
  • Data: 28-feb-2020


  • Resumen Espectáculo
  •  

    The use of biostimulants has grown in recent years and increased the productivity of important crops such as maizen. Humic substance (SH) biostimulants alter plant metabolism in different ways, but the mechanisms of action are still poorly understood. Therefore, the objective of this work was to identify the mechanisms of action of a commercial leonardite-derived humic acid biostimulant in the growth and development of maize seedlings. Maize plants were grown in nutrient solution with a commercial humic acid biostimulant extracted from leonardite and without the biostimulant (negative control). Initially root and shoot were collected separately, RNA extracted and libraries sequenced on the Illumina HISeq 2500 Paired-End 2x150pb platform generating a total of 264.3 million readings, which 92.8% were mapped in a unique position in the B73 maize reference reference (AGPv4 version). Transcriptomic analysis identified a total of 370 differentially expressed genes (DEGs) in root and 809 in shoots from treated and control plants (FDR <0.05). Overexpressed genes in the root were linked to amino acid and ketone metabolic processes, chitin catabolism, ionic copper and iron transport mechanisms, while overexpressed genes in the shoot were associated with photosynthetic processes, signaling and biosynthesis of different phytohormones. Gene clusters were identified that regulate the same biological functions in both tissues, which are related to protection against different biotic and abiotic stresses, lipid metabolism, synthesis and degradation of cell wall components, biosynthesis of secondary metabolites, nitrate response and transport. On the other hand, the biostimulant suppressed genes related to high light intensity response (photoinhibition), saline stress response and heat response. The ZmLAX3, ZmPIN9 and ZmNRT1.1 genes were also activated when plants were treated with biostimulant. The biostimulant led to an increase in the root surface and consequently to a higher plant dry weight, but there was no change in the macro and micronutrient content. ATPase activity was 2.6-fold higher in biostimulant-treated plants, which also presented higher rhizospheric acidification than the control. In addition, the biostimulant presented high indole-3-acetic acid (AIA) content. Commercial humic

     

    ix

     

    acid-based biostimulant extracted from leonardite promoted an higher plant growth by increasing the root surface, activating mechanisms related to hormone synthesis, especially AIA, H+-ATPase activity and acidification by proton extrusion ( H+) in the apoplast, which favor cell expansion, increased the expression of nitrate transporters, genes related to secondary metabolism, cell wall and in the shoot increase the expression of photosynthetic genes. Therefore, analysis of gene expression, biochemistry and physiology increased the understanding of the mode of action of humic acid-based biostimulant, and this information may be used in the future to establish regulatory guidelines.

     

     

     


2
  • JOAO PAULO RODRIGUES VIANA
  • DIRECT AND INDIRECT MECHANISMS OF THE BASIL (Ocimum basilicum) IN REDUCING THE ATTACK OF HERBIVORES TO ADJACENT PLANTS OF Brassica oleracea.

  • Líder : WELLINGTON GARCIA DE CAMPOS
  • MIEMBROS DE LA BANCA :
  • ANA PAULA MADUREIRA
  • LIVIA MENDES DE CARVALHO SILVA
  • LUÍS CLÁUDIO PATERNO SILVEIRA
  • ROGERIO MARTINS MAURICIO
  • WELLINGTON GARCIA DE CAMPOS
  • Data: 10-mar-2020


  • Resumen Espectáculo
  • The use of companion plants in the crop can increase the presence of enemies
    and indirectly mediate population control of insect pests. These
    plants can also have a direct repellent effect on undesirable insects. This study discriminated against these mechanisms of basil (Ocimum basilicum) in reducing the attack of Brevycorine brassicae to neighboring plants of Brassica oleraceae. In the field, basil increased the abundance of
    aphid predators and decreased B. brassicae in adjacent plants.
    Controlled experiments have shown that basil repels or confuses
    oviposition of Plutella xylsotella and the choice of the plant by B. brassicae. Its effect
    repellent, in the absence of natural enemies, generated a population pattern of B.
    brassicae similar to that observed in the field. A possible role of enemies
    natural, mediated by basil, was not exclusive in promoting the reduction
    in abundance of B. brassicae. The repellency or confusion of the herbivore by
    companion plant had an evident and primordial function. Basil demonstrated
    potential for use as a companion plant in brassica crops, once
    that was able to mitigate the attack of B. brassicae, either by indirect effect, via
    increase in predators, or directly and mainly, by repellency.
    The use of companion plants in the crop can increase the presence of enemies
    and indirectly mediate population control of insect pests. These
    plants
    also
    can
    exercise
    one
    It is made
    repellent
    direct
    about
    insects
    undesirable. This study discriminated against these mechanisms of basil (Ocimum
    basilicum) in reducing the attack of Brevycorine brassicae to neighboring plants of
    Brassica
    oleraceae.
    In
    field,
    O
    basil
    increased
    The
    abundance
    in
    aphid predators and decreased B. brassicae in adjacent plants.
    Controlled experiments have shown that basil repels or confuses
    oviposition of Plutella xylsotella and the choice of the plant by B. brassicae. Its effect
    repellent, in the absence of natural enemies, generated a population pattern of B.
    brassicae similar to that observed in the field. A possible role of enemies
    natural, mediated by basil, was not exclusive in promoting the reduction
    in abundance of B. brassicae. The repellency or confusion of the herbivore by
    companion plant had an evident and primordial function. Basil demonstrated
    potential for use as a companion plant in brassica crops, once
    that was able to mitigate the attack of B. brassicae, either by indirect effect, via
    increase in predators, or directly and mainly, by repellency.

3
  • GUILHERME FREDERICO PEDRA PONCIANO
  • Useofmodelinput-state-outputforto assessThesustainability of a silvopastoral system

  • Líder : FENI DALANO ROOSEVELT AGOSTINHO
  • MIEMBROS DE LA BANCA :
  • ANTONIO MARCIO RODRIGUES
  • FENI DALANO ROOSEVELT AGOSTINHO
  • GISELE SILVA NOGUEIRA
  • JOAO DOMINGOS SCALON
  • MARIO ANTONIO DUARTE
  • Data: 20-mar-2020


  • Resumen Espectáculo
  • The livestock activity has a global economic importance, however it is
    constantly associated with degradations and environmental impacts. Combining
    this with the future need to increase productivity to meet consumer demand,
    assessing the sustainability of these systems is of great importance. In addition
    to the sustainability assessment, it is interesting to discuss and indicate better
    ways of handling the system, which in the case of livestock use free inputs
    provided by nature. This study aims to bring together environmental accounting
    and emissions inventory methodologies, using the input-state-output model.
    Environmental accounting was defined as the input parameters of the system,
    the advantage of this methodology is that it converts all energy flows in the
    system into a common unit, in addition to quantifying and valuing the natural
    resources intrinsic to the system. The methodology also provides indexes that
    summarize the relationships, dependencies, and impacts of the use of inputs.
    The state of the system was defined as the profitability and the output was
    estimated as the CO2eq emission from the system, considering the direct
    emissions from the system and the previous emissions for the generation of the
    used inputs. The indices of emergy yield rate (EYR), environmental load rate
    (ELR) and sustainability index (ESI) were used to compose a radar diagram, in
    order to express the sustainability of the system, regarding the inputs. The
    proposed sustainability assessment model was applied to a farm (Fazenda
    Monalisa - FML), located in the legal Amazon region (Brazil) and its
    management is via agroecological practices, which in livestock can be
    summarized in silvopastoral management (SPS), which uses vegetation to
    compose the system, such as hedges, trees scattered in the pasture that serve
    as a source of shade, in addition to being able to serve as food for the animal
    reducing the dependence on external inputs. The advantages that the SPS
    provides for livestock were evidenced mainly by analyzing the radar diagram
    and comparing it with data from similar systems in the literature. The FML
    presented an EYR of 2.99, an ELR of 0.53 and an ESI of 5.61, the indices were
    one of the best found for the livestock system, in the comparisons between the
    systems and these indices it was possible to verify the positive influences that
    the portion cause in the system. FML's profitability was 3.17, which is quite
    satisfactory for this financial activity. The CO2eq emission in the system was 1.72
    tons per hectare in the year. In view of all the benefits and influences caused by
    the plant plot in the system, the net carbon potential was calculated, considering
    the CO2 absorption potential by the vegetation, the calculations showed the
    potential that the system has to be a gas mitigator, all thanks to the plant plot
    that is a fundamental part of the system. The SPS influences on the livestock
    system are so many that with everything that was discussed in the study it also
    provided the basis for the discussion on the item biodiversity within
    environmental accounting.

4
  • RAFAEL SANDIN RIBEIRO
  • Enteric methane in tropical conditions: particle size and prediction equations

  • Líder : ROGERIO MARTINS MAURICIO
  • MIEMBROS DE LA BANCA :
  • Adibe Luiz Abdalla
  • JOÃO PAULO PACHECO RODRIGUES
  • LUIZ GUSTAVO RIBEIRO PEREIRA
  • ROGERIO MARTINS MAURICIO
  • THIERRY RIBEIRO TOMICH
  • Data: 22-abr-2020


  • Resumen Espectáculo
  • xxxxx

5
  • RAFAEL SANDIN RIBEIRO
  • Enteric methane in tropical conditions: particle size and prediction equations

  • Líder : ROGERIO MARTINS MAURICIO
  • MIEMBROS DE LA BANCA :
  • Adibe Luiz Abdalla
  • JOÃO PAULO PACHECO RODRIGUES
  • LUIZ GUSTAVO RIBEIRO PEREIRA
  • ROGERIO MARTINS MAURICIO
  • THIERRY RIBEIRO TOMICH
  • Data: 22-abr-2020


  • Resumen Espectáculo
  • xxxxx

6
  • RAFAEL SANDIN RIBEIRO
  • EMISSÃO DE METANO ENTÉRICO POR BOVINOS EM CONDIÇÕES TROPICAIS: EFEITO DO TAMANHO DE PARTÍCULAS E EQUAÇÕES DE PREDIÇÃO

  • Líder : ROGERIO MARTINS MAURICIO
  • MIEMBROS DE LA BANCA :
  • Adibe Luiz Abdalla
  • JOÃO PAULO PACHECO RODRIGUES
  • LUIZ GUSTAVO RIBEIRO PEREIRA
  • ROGERIO MARTINS MAURICIO
  • THIERRY RIBEIRO TOMICH
  • Data: 22-abr-2020


  • Resumen Espectáculo
  • -----

7
  • SIMARA DA SILVA LOPES
  • ANÁLISE FUNCIONAL DO GENE DE ARROZ PHOSPHORUS-STARVATION TOLERANCE 1 (PSTOL 1) E DE SEUS HOMÓLOGOS DE MILHO E SORGO EM PLANTAS TRANSGÊNICAS DE TABACO

  • Líder : SYLVIA MORAIS DE SOUSA TINOCO
  • MIEMBROS DE LA BANCA :
  • ANDREA ALMEIDA CARNEIRO
  • CLAUDIA TEIXEIRA GUIMARAES
  • EVANDRO NOVAES
  • JONI ESROM LIMA
  • JURANDIR VIEIRA DE MAGALHÃES
  • LEONARDO LUCAS CARNEVALLI DIAS
  • SYLVIA MORAIS DE SOUSA TINOCO
  • Data: 29-may-2020


  • Resumen Espectáculo
  • ANÁLISE FUNCIONAL DO GENE DE ARROZ PHOSPHORUS-STARVATION TOLERANCE 1 (PSTOL 1) E DE SEUS HOMÓLOGOS DE MILHO E SORGO EM PLANTAS TRANSGÊNICAS DE TABACO

8
  • SAMYRA GIAROLA CECILIO
  • INFLUENCE OF CHLORIDE REGULATORY MECHANISMS ONNON-SYNAPTIC EPILEPTIFORM ACTIVITIES

  • Líder : ANTONIO CARLOS GUIMARAES DE ALMEIDA
  • MIEMBROS DE LA BANCA :
  • ANTONIO CARLOS GUIMARAES DE ALMEIDA
  • ANTONIO MARCIO RODRIGUES
  • CARLA ALESSANDRA SCORZA BAHI
  • FULVIO ALEXANDRE SCORZA
  • LUIZ EDUARDO CANTON SANTOS
  • RICARDO MARIO ARIDA
  • Data: 24-ago-2020


  • Resumen Espectáculo
  • Chloride is the most abundant physiological anion and it determines elementary biological functions. Its intracellular levels result from a balance established by channels, exchangers and transporters present in the plasma membrane. Changes  in the balance of this ion occur in pathological conditions such as epilepsy, in which the accumulation of intracellular chloride in neurons of the central nervous system (CNS) is related to the induction and sustentation of epileptiform activities. Dysfunctions in cation-chloride cotransporters (CCC’s) have been identified as the main responsible for this accumulation. Previous experiments have shown that perfusion with low extracellular chloride ([Cl-]o) (7 mM) reversibly blocks spontaneous hyper-synchronous discharges induced by high potassium [K+]o without reducing synaptic hyperexcitability. Furthermore, this anticonvulsant effect is similar to that of furosemide and, therefore, mediated by the effect on CCC`s, especially NKCC1 and KCC2. Thus, the aim of the present study was to investigate the extracellular depletion of the chloride ion as well as the influence of its regulatory mechanisms on non-synaptic epileptiform activities (AENS) induced in hippocampal slices of Wistar rats using the zero calcium and high [K+]o model (8 mM). Extracellular electrophysiological recordings in the dentate gyrus allowed us to evaluate the effect of these mechanisms on the AENS. In addition to sodium gluconate for equimolar replacement of the chloride ion, the use of furosemide (5 mM), bumetanide (12.5 μM), VU 0463271 (10 μM), acetazolamide (100 μM) and amiloride (0.5 mM), alone or combined, composed eighteen experimental groups. Programs developed on the MATLAB platform made it possible to record the extracellular electrical potential, and the parameters event duration (ED), interval between events (IE), amplitude of population spikes, (PS's), maximum amplitude (max. Amp) and DC component were quantified. The low extracellular [Cl-] maneuver did not cause suppression of the AENS, an effect obtained during the maneuver with furosemide. Conversely, the reduction in CCC activity caused by the low [Cl-] maneuver promoted greater neuronal excitability. Likewise, the selective blocking of NKCC1 with bumetanide, KCC2 with VU 0463271 or the simultaneous addition of both inhibitors (bumetanide + VU) did not suppress AENS, demonstrating an additional effect of furosemide on AENS, apart from the effect on cation-chloride cotransporters. Based on data from the literature, according to which the diuretic also acts on carbonic anhydrase and  the sodium-independent chloride-bicarbonate exchanger (AE3), the next experimental step was to investigate the effect of the diuretic on pH, mechanisms that are also influenced by intracellular chloride concentrations. For this, the change in the pH of the extracellular medium was analyzed, maintaining the blocking of the cotransport mechanisms, with the use of furosemide and low [Cl-]o. By making the pH of the zero-calcium high-potassium solution more basic, around 7.55, the return of epileptiform activities was observed, showing that the blockage exerted by furosemide causes acidification of the extracellular medium. After elucidating the activity of furosemide on pH, the investigation of the effect of blocking mechanisms that act on neuronal pH in AENS was initiated, in order to identify the possible target(s) of this diuretic and, consequently, the mechanisms that make it possible to sustain epileptiform activities. However, the use of acetazolamide (ACZM), a carbonic anhydrase inhibitor, or other combined CCC inhibition maneuvers and neuronal pH regulatory mechanisms, such as bumetanide + VU + ACZM and zero [Cl-]o + ACZM did not promote the suppression of epileptiform activities. The




    condition of zero [Cl-]o and zero [NaHCO3]o, however, which mimics AE3 block, unlike the other groups, showed a progressive reduction in epileptiform events, with a tendency towards suppression. Thus, the additional effect of a pH-regulating mechanism that has not yet been investigated, the Na+/H+ exchanger (NHE), was also evaluated, since during the ictal period, with the accumulation of intracellular sodium, an inversion of its performance is suggested, with the intrusion of a H+ ion and extrusion of a Na+ ion. The combination of the maneuvers resulted in the appearance of regular oscillations at the baseline with subsequent suppression of the AENS. In short, it was possible to observe that the suppression of AENS was only possible due to a combined action on different non-synaptic mechanisms, in this case, cation-chloride cotransporters and NHE exchanger, which would also influence the Na+/K+ ATPase activity. Still, the sodium/bicarbonate cotransporter (NBC) is a possible target of the diuretic, since it maintains the alkalinity of the extracellular medium, and, consequently, neuronal hyperexcitability. However, there is no specific blocker for this transport mechanism yet.


9
  • DELMO BENEDITO SILVA
  • EFFECTS OF THE [Mg2+] REDUCTION DURINGINDUCTION OF NON-SYNAPTIC EPILLETIFORM ACTIVITIES

  • Líder : ANTONIO MARCIO RODRIGUES
  • MIEMBROS DE LA BANCA :
  • ANTONIO CARLOS GUIMARAES DE ALMEIDA
  • ANTONIO MARCIO RODRIGUES
  • ANTONIO MAURICIO FERREIRA LEITE MIRANDA DE SA
  • DIEGO BASILE COLUGNATI
  • GISELE SILVA NOGUEIRA
  • Data: 29-dic-2020


  • Resumen Espectáculo
  • Magnesium (Mg2+) is an essential mineral withcrucial role in several cellular functions and corresponds to the second most abundant intracellular cation. During the induction of epileptiform activities,reductionsin extracellular concentrations of Mg2+([Mg2+]) and calcium wereobserved. Most studies have focused on the effect of Mg2+on synaptic epileptiform activities;however,data on the effect of [Mg2+] reduction on non-synaptic epileptiform activities (NSEA) are scarce. Thegoal of the present work was, using experimental maneuvers and computational simulations, to understand the possible effects of [Mg2+] variation on NSEA. As an experimental protocol, NSEA were induced in rat hippocampal dentate gyrus (DG) hippocampus, using a bath solution containing high-K+and zero-added-Ca2+. Computer simulations were performed using a mathematical model that represents electrochemical characteristics of the tissue of the DG granular layer. The experimental results show that the reduction of [Mg2+] causes an increase in the duration of the ictal period anda reduction in the inter-ictal period, intensifying epileptiform discharges. The computer simulations suggest that the reduction of the Mg2+level intensifies the epileptiform discharges by a joint effect of reducing the surface charge screening and reducing the activity of the Na+/K+-ATPase.

2019
Disertaciones
1
  • RAYANNE PEREIRA DE OLIVEIRA
  • Allelic variability of the ZmMATE1 gene associated with aluminum tolerance in a panel of tropical maize lines

  • Líder : CLAUDIA TEIXEIRA GUIMARAES
  • MIEMBROS DE LA BANCA :
  • CLAUDIA TEIXEIRA GUIMARAES
  • JURANDIR VIEIRA DE MAGALHÃES
  • WAGNER LUIZ ARAUJO
  • Data: 28-mar-2019


  • Resumen Espectáculo
  • Aluminum (Al) toxicity is a limiting factor for crop yield on acid soils, which covers approximately 50% of the world arable lands. Al tolerance is a complex trait in maize (Zea mays L.), where until now, only the ZmMATE1 was characterized as a gene responsible for part of this tolerance, via citrate exudation in root apices. The allelic form of the ZmMATE1 gene that confers Al tolerance is organized in three tandem copies and was associated with the high levels of gene expression in Cateto Al237. The allele present in the line L53, highly sensitive to Al, is organized in a single copy and was associated with low expression of ZmMATE1. A single nucleotide polymorphism (SNP) located in the promoter region of the ZmMATE1 gene was shared with a small group of lines with high expression of this gene, being a first evidence of the association between this polymorphism and gene expression. Thus, the objective of this study was to investigate the frequency and distribution of the three copies and the SNP in the promoter region of ZmMATE1 in a tropical maize panel of wide diversity, aiming to consolidate the inferences about the effect of these polymorphisms in the expression of ZmMATE1 and in the Al tolerance. The panel was composed of 60 maize inbred lines, selected from 363 lines of Embrapa and 21 of CIMMYT (International Center for Improvement of Maize and Wheat), which were characterized for the copy number variation, the SNP in the promoter region and the gene expression of ZmMATE1, in addition to the Al tolerance in nutrient solution. Three new lines containing the ZmMATE1 gene in triplicate were identified in the panel, of which two were from Brazil and one from Colombia. All maize lines with three copies of ZmMATE1 showed the T allele, high gene expression and tolerance to Al from high to intermediate. The T allele was associated with an increase in the expression level of the ZmMATE1 gene, which was originated probably in the Composto Tuxpeño and in the phylogenetic group shared with the lines derived from Cateto landrace. Two lines highly Al tolerant did not have the superior allele of ZmMATE1, confirming the existence of different Al tolerance mechanisms in maize. Thus, the wide genetic and

     

    phenotypic variability within the tropical maize panel allowed the identification of new combinations of alleles conferring high expression of ZmMATE1, in addition to new genes, which may be pyramided to develop cultivars with superior levels of Al tolerance.

     


2
  • CAMILA CRISTINA VIEIRA VELLOSO
  • CHARACTERIZATION OF Bacillus STRAINS AND EVALUATION OF THEIR GROWTH PROMOTING POTENTIAL IN MAIZE GENOTYPES

  • Líder : SYLVIA MORAIS DE SOUSA TINOCO
  • MIEMBROS DE LA BANCA :
  • CHRISTIANE ABREU DE OLIVEIRA PAIVA
  • SYLVIA MORAIS DE SOUSA TINOCO
  • VERA LUCIA DOS SANTOS
  • Data: 29-jul-2019


  • Resumen Espectáculo
  • Several biotic and abiotic factors contribute to lower maize productivity in Brazil. The use of plant growth promoting bacteria has been shown to be an efficient and sustainable alternative to mitigate these problems. The objective of this work was to characterize the genome of two Bacillus strains and to evaluate their potential for promoting growth in maize genotypes. Illumina HiSeq 4000 sequencing and genome analysis of the two Bacillus strains (B116 and B119) from Collection of Multifunctional Microorganisms of Embrapa Maize and Sorghum showed the presence of genes related to endospore formation, chemotaxis, motility, competition in the rhizosphere and several mechanisms of plant growth promotion in the two bacterial strains. Bacterial strain B116 was classified as B. thuringiensis and as B119 as B. megaterium. The two Bacillus were able to produce exopolysaccharides (EPS) and fix nitrogen. However, B. megaterium produced higher amounts of IAA and siderophores, whereas

    B. thuringiensis was characterized as the best biofilm producer and was capable to solubilize more phosphate. Strain B116 reached maximum phosphatase production in 48 h, while B119 required 96 h to reach the same point. In addition, maize seedlings inoculated and submitted to drought stress had a greater reduction of fine root surface area and an increase of thicker roots, which resulted in a higher root dry weight. The root remodeling led to a lower total dry weight loss when compared to the un-inoculated control. The inoculation with the Bacillus strains increased the dry weight and the root system of 16 from the 21 maize genotypes tested, while four genotypes showed negative responses and one was not affected, indicating that the interaction between plants and bacteria was genotype-dependent. The in vitro characterization and hydroponic results corroborated the presence of the genes that compose the different metabolic pathways related to growth promotion and showed that the association of these Bacillus strains can benefit plants, as a result of the root modulation, nutrient acquisition and greater resistance to stress. The information obtained in this work allowed the characterization of growth promotion mechanisms from two Bacillus strains, maximizing the understanding of plant-bacterial interaction.

     

     

     

     


3
  • ARTHUR CARVALHO PIRES
  • DEVELOPMENT OF AN AUTOMATED SYSTEM FOR THE CONTROL OF FLOW AND EXCHANGE OF SOLUTIONS IN ELECTROPHYSIOLOGICAL REGISTRATION SYSTEMS OF HIPOCAMPAAL SLICES.

  • Líder : ANTONIO CARLOS GUIMARAES DE ALMEIDA
  • MIEMBROS DE LA BANCA :
  • ANTONIO CARLOS GUIMARAES DE ALMEIDA
  • LUIZ EDUARDO CANTON SANTOS
  • ANTONIO MARCIO RODRIGUES
  • DAMIEN THOMAS DEPANNEMAECKER
  • Data: 09-dic-2019


  • Resumen Espectáculo
  • Gravity perfusion systems have a flow dependent on the difference between the levels of the supply reservoir and the infusion point. In electrophysiology experiments in which such systems are used, it is usual to periodically adjust the flow and exchange solutions manually. However, the handling of regulators for flow control, and manual valves for changing solutions during signal acquisition can cause mechanical and electrical interference from different sources, impairing the signal/noise ratio. This work describes the materials and procedures used in the construction of an automatic flow control system with gradual adjustments and exchange of perfusion solutions in electrophysiological experiments with brain slices. And it aims to reduce, as much as possible, the artifacts originating from the procedures for closing and opening records of each solution used. An optical sensor for drop instrumentation and two flow actuating devices were developed. The exchange device consists of solenoid valves and pumps to recirculate the solution. A Human Machine Interface (HMI) was developed for user interaction with the system, and an Atmel2550 microcontroller was used as the control unit for the entire set. All manufactured devices were tested in electrophysiological recording systems for the acquisition of electrical signals in hippocampal slices through an interface chamber. The system proved to be an effective tool for the standardization of experiments, in addition, the results also showed a decrease in flow error and attenuation of interferences in the procedures for exchanging perfusion solutions.


Tesis
1
  • BÁRBARA FRANCA NEGRI
  • Functional characterization of maize genes homologous to Phosphorus Starvation Tolerance 1, responsible for increasing a root surface, phosphorusacquisition and grain yield in rice.

  • Líder : SYLVIA MORAIS DE SOUSA TINOCO
  • MIEMBROS DE LA BANCA :
  • LUIZ EDUARDO VIEIRA DEL BEM
  • CLAUDIA TEIXEIRA GUIMARAES
  • JANAÍNA DE OLIVEIRA MELO
  • LEONARDO LUCAS CARNEVALLI DIAS
  • SYLVIA MORAIS DE SOUSA TINOCO
  • Data: 27-mar-2019


  • Resumen Espectáculo
  • Low phosphorus (P) availability is a major constraint for cereal productivity in tropical soils. Modifications in root morphology are the major adaptation mechanism plant developed under P starvation, once provide greater nutrient acquisition through larger soil exploration. The Phosphorus-starvation tolerance 1(PSTOL1) gene was identified as responsible for the locus Phosphorus Uptake 1(Pup1), and encodes a protein kinase responsible for increased early root growth, P uptake, and grain yield in rice. Sorghum (Sorghum bicolor, SbPSTOL1) and maize (Zea mays, ZmPSTOL1) homologs to OsPSTOL1were identified via associationand quantitative trait loci (QTLs)mappingof traits related toroot system morphology modulationand yield increased under low P.This study aimed to characterize rice OsPSTOL1, sorghum (Sb03g006765, Sb03g031690and Sb07g002840) and maize(ZmPSTOL1_8.05, ZmPSTOL1_3.06and ZmPSTOL1_8.02)genes.For this, maize plants weretransformedvia Agrobacterium tumefacienswithOsPSTOL1,SbPSTOL1and ZmPSTOL1genes.The events were analyzed in nutrient solution under low P and in soil under low and high P.OsPSTOL1and SbPSTOL1eventspresentedan increase of superfine roots, which led to an increase in root surface area and plant biomass, both in the growing chamber and in the greenhouseconditions.Events overexpressing the ZmPSTOL1genes did not present significant differences in relation to the wild typeB104. In addition, the detailed characterization of the ZmPSTOL1_3.06maize gene was performed.ZmPSTOL1_3.06expression occurred preferentially in the differentiation zone of the primary root, more precisely in the root hairsof the P-efficientlineand was modulated by Pavailability.The presence of the binding motifs related to thePacquisition (ZmPHR1)and the formation of root hairs(ZmLRL5) in ZmPSTOL1_3.06promoter region,together with the fact that the transgenic plants under itspromoter showed higher GUS activity under low P and in root hairs, indicated a potential relationship between these transcription factors and 15ZmPSTOL1_3.06.Moreover, transgenic plants overexpressing ZmPSTOL1_3.06altered root hairselongation the expression of genes related to their formation.Ourresults suggestedso far,that the PSTOL1 genes have a more general role in the root systemdevelopment, which results inanincreased P efficiency, which may benefit cereal production

2
  • DENISE PACHECO DOS REIS
  • PRODUCTIVITY, ACCUMULATION OF NUTRIENTS AND BACTERIAL DIVERSITY OF MAIZE GENOTYPES RHYPHOSIS IN RESPONSE TO INOCULATION WITH Azospirillumbrasilense.

  • Líder : IVANILDO EVODIO MARRIEL
  • MIEMBROS DE LA BANCA :
  • CHRISTIANE ABREU DE OLIVEIRA PAIVA
  • LAURO JOSE MOREIRA GUIMARAES
  • FLAVIA FERREIRA MENDES GUIMARAES
  • FREDERICK MENDES AGUIAR
  • IVANILDO EVODIO MARRIEL
  • MARCIA REGINA DA COSTA
  • Data: 29-mar-2019


  • Resumen Espectáculo
  • The use of synthetic inputs is of fundamental importance for the productive potential achieved by modern agriculture. However, the intensive use of this input can compromise agricultural sustainability in the environmental dimension. The practice of inoculation with a biological product based on bacteria that can fix nitrogen has shown to be a promising and sustainable option, to improve production, reduce production costs and low ecological impact in agroecosystems. These bacteria colonize the rhizosphere and internal plant tissues promoting benefits to crops and are in constant interactions with autochthonous microorganisms. The objective of this scenario was to evaluate the effect of inoculation with Azospirillumbrasilense on the bacterial diversity of the rhizosphere of different corn hybrids, nutrient accumulation, and grain yield. Different maize genotypes inoculated with a mixture of three strains of Azospirillum brasilense were used. Bacterial diversity was estimated in samples of total DNA extracted from rhizospheric soil after second-generation sequencing. The nutrient accumulation and productivity were measured in the flowering phase and at the end of the cycle, respectively. The results of the metagenomic analysis showed that the composition and structure vary according to the genotype of the plant and the inoculant, but there are changes mainly in the abundance of some phyla and genera present in the soil samples. There were gains in grain yield and nutrient accumulation as a function of the inoculation of the evaluated genotypes. Therefore, it is concluded that the formation of the structure and composition of the bacterial community of the rhizospheric soil of the plants was dependent on the inoculation with Azospirillumbrasilense and the plant genotype. The agronomic characteristics of corn (Productivity and accumulation of nutrients) are influenced by the use of inoculants

3
  • ALISSON DE OLIVEIRA
  • DIVERSIDADE E DISTRIBUIÇÃO SAZONAL DE ANFÍBIOS E RÉPTEIS EM FRAGMENTOS DE MATA NATIVA E PLANTIOS DE Eucalyptus urograndis

  • Líder : WELLINGTON GARCIA DE CAMPOS
  • MIEMBROS DE LA BANCA :
  • BERNADETE MARIA DE SOUSA
  • IVAN CARLOS DOS SANTOS
  • MARIA GORETI ALMEIDA OLIVEIRA
  • ROGERIO MARTINS MAURICIO
  • WELLINGTON GARCIA DE CAMPOS
  • Data: 21-oct-2019


  • Resumen Espectáculo
  • -

4
  • Fabrício Neves Mendonca
  • PHYSICAL EXERCISE AND EPILEPSY: NEUROPROTECTION AND IMPROVEMENT OF MECHANISMS FOR MAINTENANCE OF NEURONAL IONIC HOMEOSTASIS

  • Líder : ANTONIO CARLOS GUIMARAES DE ALMEIDA
  • MIEMBROS DE LA BANCA :
  • ANTONIO CARLOS GUIMARAES DE ALMEIDA
  • ANTONIO MARCIO RODRIGUES
  • CARLA ALESSANDRA SCORZA BAHI
  • FULVIO ALEXANDRE SCORZA
  • RICARDO MARIO ARIDA
  • Data: 17-dic-2019


  • Resumen Espectáculo
  • Epilepsy is a neurological disorder that can be triggered by a neurological insult that induces a subsequent hyperexcitability of the nervous tissue, leading to the occurrence of spontaneous seizures. Physical exercise has been shown to promote improvements in the brain of the individual with epilepsy, exerting a neuroprotective effect. This study evaluated the potential of physical exercise during adult life as a therapy for treatment of victims of neurological injury during brain development. The results found here demonstrate that physical exercise may be able to produce protective effects both on the integrity of the nervous tissue and on some of the ionic homeostasis mechanisms affected by the neurological insult promoted by the status epilepticus, restoring the electrophysiological excitability of populations of neurons to a similar to that seen in intact tissue.

     


2018
Disertaciones
1
  • VITÓRIA PALHARES RIBEIRO
  • MIXED AND SIMPLE INOCULATION WITH AZOSPIRILLUM BRASILENSE AND ACILLUS SP. IN CORN PLANTS: DEVELOPMENT OF TECHNOLOGIES FOR SUSTAINABLE AGRICULTURAL SYSTEMS.

  • Líder : IVANILDO EVODIO MARRIEL
  • MIEMBROS DE LA BANCA :
  • IVANILDO EVODIO MARRIEL
  • SYLVIA MORAIS DE SOUSA TINOCO
  • AMANDA APARECIDA DE OLIVEIRA NEVES VIANA
  • Data: 28-feb-2018


  • Resumen Espectáculo
  • The corn (Zea mays L.) crop occupies a prominent position in the world’s cereal
    production. The high crop yield is the result of high nitrogen and phosphorus inputs,
    which have negative impact on the environment and at high costs. Therefore, it is
    important to adopt agricultural management practices using plant growth-promoting
    microorganisms to reduce the use of chemical fertilizers in agriculture. The objective
    of this work was to evaluate the effect of simple and mixed inoculation of two strains
    of Azospirillum brasilense (2142 and 1626) and two Bacillus sp. (B2084 and B116)
    on the growth and root morphology of maize under in vitro conditions, in a growth
    chamber and in the greenhouse. The compatibility and synergism among the strains,
    phosphate solubilising ability and Indole Acetic Acid (IAA) production were also
    evaluated in vitro. The compatibility between strains was estimated by direct
    confrontation tests, solubilized P and mineralized P were determined after nine days
    of incubation at 28± 2ºC, and the soluble P was determined by colorimetric method.
    The efficiency of the inoculants was evaluated by 11 treatments (4 simple, 6 mixtures,
    and 1 control without inoculant) carried out in a floating system. The seedlings were
    acclimatized for seven days in Hoagland nutrient solution (half strength), followed by
    inoculation of the roots and incubation period of 6 hours. The total dry weight of shoot,
    roots and plants were evaluated, and root morphology was determined by the image
    capture system and analysis with WinRhizo software. The same treatments were
    reproduced in a greenhouse test with the addition of two types of phosphate fertilizers
    (Araxá rock phosphate and Triple Superphosphate). The results showed the positive
    effects of the interactions between and among strains. In vitro, the mixed treatment
    consisting of 2 strains of A. brasilense (2142x1626) and the simple treatment with
    Bacillus sp. showed the highest release of soluble P for the three sources tested (P-Fe,
    P-Ca and P-phytate). These treatments also resulted in higher values of total root
    length, total aerial surface, root surface area (AS) with diameters 0> AS <1.0 mm and
    1.0> AS <2.0 mm, Root, Shoot and Seedlings dry weights, as well as an increased production of IAA. In the greenhouse experiment, significant differences were
    observed for shoot dry weight and total dry weight of the plant when the corn was
    inoculated with the two A. brasilense strains (1626x2142) and the triple
    superphosphate fertilizer. The results demonstrated the potential of Azospirillum
    brasilense and Bacillus sp. for inoculant formulations aiming the increase the corn
    plant biomass. Field experiments and studies on nutrient accumulation in maize plants
    are necessary to validate the present results.

Tesis
1
  • ANA PAULA CAMPOS DE CARVALHO
  • EFFECTS OF SOIL USE ON THE STRUCTURE AND FUNCTIONING OF THEBIOFILM IN RIACHOS IN THE MATA ATLÂNTICA TRANSITION - CERRADO

  • Líder : BJORN GUCKER
  • MIEMBROS DE LA BANCA :
  • ANDRE BATISTA DE NEGREIROS
  • BJORN GUCKER
  • CLEBER CUNHA FIGUEREDO
  • DAVI GASPARINI FERNANDES CUNHA
  • IOLA GONCALVES BOECHAT
  • Data: 16-feb-2018


  • Resumen Espectáculo
  • Modifications on natural landscape can affect lotic ecosystems due to the close relationship that
    streams and rivers have with the surrounding landscape, altering water quality, structure and
    functioning. Different techniques are used to assess the impacts of land use on aquatic systems
    and a large number of bioindicators have been used to determine the effectiveness of different
    approaches in river management. Stream biofilm is one of the major biological components of
    metabolism in lotic ecosystems, it is formed by autotrophic and heterotrophic cells involved an
    extracellular matrix growing on a solid substrate.
    These organisms are sensitive to environmental changes and they can be quickly sampled and have a range of attributes that canbe measured quantitatively. The aim of this study was to evaluate the water quality, the structure of stream biofilm community as well as whole-stream metabolism in streams under differentimpacts: natural, pasture, agriculture and urbanized, located in a transition area between
    Atlantic Forest and Cerrado, in Minas Gerais Federal State. The study consisted of an annual
    sampling in six streams located in the Rio das Mortes basin between June 2014 and July 2015
    and the results were divided into three chapters. In chapter 1, water quality, autotrophic biomass
    (measured in situ, with a BenthoTorch probe, and after extraction of chlorophyll-a in ethanol)
    and biofilm stoichiometry were evaluated. The water quality is altered by the different land use;
    however, the impact on biofilm may not be so easily identified with the evaluation of simple
    variables such as total nutrients concentrations or biomass. In Chapter 2, the biofilm community
    structure was evaluated from microscopic and molecular techniques (Terminal Restriction Fragment Length Polymorphism, T-RFLP). A distance-based redundancy analysis (dbRDA)
    indicated that algal community, identified by light microscopy, was influenced by land use,
    which explains 46.3% of the community variability and separated all the streams, indicating
    that the specificities of each habitat such as hydrodynamics, nutrient concentrations, light and
    temperature conditions, substrate and biological interactions may influence the biofilm
    colonization. The dbRDA analyzes, for communities analyzed via T-RFLP, explained less than
    20% in the variability of the data and were significant for algae, bacteria and fungi, but not for ciliates. A comparison of algal community evaluated by microscopy and molecular did not
    show a significant relationship between the techniques and, although T-RFLP is commonly
    reported as a rapid method for biofilm analysis, in this study, microscopy proved more robust
    in evaluate the structure of algal community in different streams. In chapter 3, whole-stream
    metabolism was evaluated through gross primary production (GPP) and community respiration
    (CR) rates, which are largely performed by biofilm in streams without large macrophyte
    biomass or filamentous algae. The streams were characterized by a heterotrophic state. The
    m-2d-1) highest average GPP rate was found in an urban stream (5. 25 g OD
    differing from the other streams. Natural and agricultural streams did not differ in relation to their GPP. CR rates  were higher in the two urban streams differing from the others. Natural and agricultural streams
    did not differ between them. CR rate found in these urban streams was the highest among those
    m-2d-1), already reported in the literature (70 g OD
    suggesting that carbon (C) inputs associated
    with domestic sewage are more labile than naturally transported organic matter and thus, it is
    considered an important C source allowing CR increases. In turn, PPB:RC was less than 0.5
    which characterizes a heterotrophic state in all of studied streams suggesting that the metabolic
    network in these streams is supported mainly by non-native sources. Low GPP rates in
    m-2d-1) pasture/agriculture streams (0.3 g OD
    may be related to substrate characteristics with thin and unstable sediment considered poor for algal colonization, as well as cattle trampling
    may have contributed to a greater water turbidity. On the other hand, allochthonous organic
    matter reduction received by the agricultural streams, due to clear canopy, seems to have
    influenced the CR in these streams. These results indicate that even though the metabolic rates
    in these different streams (natural and agricultural) have been similar, changes in the
    functioning systems can occur due to the differences in autotrophic communities of each stream.
    Finally, the results of this study indicate that the community structure of biofilm streams is
    considered an important bioindicator of water quality and ecosystem functioning in Atlantic
    Forest and Cerrado transition streams. With this data, management strastrategies can be applied in
    order to improve water quality and ecosystem services in streams.
     

2
  • FERNANDA MARIA DO NASCIMENTO AIHARA
  • Composting as method for the final disposalof dog and rodent carcasses.

  • Líder : WELLINGTON GARCIA DE CAMPOS
  • MIEMBROS DE LA BANCA :
  • MARCOS SANTOS ZANINI
  • ANA PAULA MADUREIRA
  • MARIA GORETI ALMEIDA OLIVEIRA
  • RICARDO MONTEIRO CORREA
  • WELLINGTON GARCIA DE CAMPOS
  • Data: 09-nov-2018


  • Resumen Espectáculo
  • Composting is a viable alternative for the disposalof carcasses, in that it is an easily implemented low-cost, non-polluting method. The organic waste so generated, generally free from pathogens, can also be used as fertilizer in agriculture. This research aimed to evaluate the composting technique, usingmethods in which material is turned and not turned, for the treatment and final disposalof dog and rodent carcasses of relevance to public health, which are generated respectively by municipal disease control and prevention agencies and by animal testingin research and education facilities. During or at the end of the composting process, the presence of the following contaminant and pathogenic microorganisms were investigated: total coliforms, thermotolerant coliforms, Escherichia coli, Salmonella spp.,and viable helminth eggs. Temperaturevariation inside the compost was monitored to evaluate its effect on the elimination of undesirable microorganisms. At the end of the processof dogs composting, the compost was evaluated for itsproperties: humidity,pH, organic carbon,C/N ratio, boron, calcium, copper, iron, nitrogen, phosphorus, magnesium, manganese, potassium and zinc; which were compared to commercial vegetable substrate Tropstrato® HT Hortaliças. Maintenance of thermophilic temperatures was important to reduce the concentrations of total coliforms, thermotolerant coliforms and viable eggs of helminths, as well as to eliminate E. coliand Salmonella spp. in dog or rodent carcasses composts. Physicochemical parameters of dog compostingpresented higher concentrationscompared to the commercial substrate Tropstrato® HT Hortaliças. In conclusion, composting, irrespective of turnings, can be recommended as it is anon-polluting method, capable of eliminating microorganisms during the final disposalof dog and rodent carcasses of relevance to public health. The method is presented as an alternative solution for biological waste generated by municipal disease control and prevention agencies and by laboratories in research institutions.

3
  • APARECIDA BEATRIZ DAS MERCES DE PAIVA MAGELA
  • MINERAL VERSUS BIOCHEMICAL INDICATORS OF QUALITYNESTRICIONAL LAGOONS AND RESERVOIRS: VARIATIONINTERNAL AND BETWEEN SYSTEMS

  • Líder : IOLA GONCALVES BOECHAT
  • MIEMBROS DE LA BANCA :
  • BJORN GUCKER
  • CLEBER CUNHA FIGUEREDO
  • DAVI GASPARINI FERNANDES CUNHA
  • IOLA GONCALVES BOECHAT
  • RICARDO MOTTA PINTO COELHO
  • Data: 06-dic-2018


  • Resumen Espectáculo
  • The nutritional quality of food available may vary according to the environmental conditions,
    which influences the efficiency of energy and matter transfer in natural and impacted lake
    systems. In this study, the questions related to the spatial, temporal and trophic variability in
    the nutritional quality of the seston of ponds and reservoirs were searched. The general aim
    was to evaluate the types of sestonic quality indicators - minerals (total nitrogen and
    phosphorus) or biochemical (total lipid and carbohydrate) indicators better respond to
    environmental changes, analyzed from the perspective of changes in water quality and
    phytoplankton communities. The general hypothesis was that the greatest responses would be
    detected for the biochemical composition. Natural variations in the mineral and biochemical
    composition of each system were analyzed (i) spatial (horizontal - three different distances of
    the margin - and vertical - two depths) and (ii) temporal (between four different sampling
    periods), as well as the variations between different trophic state systems. Five ponds and
    three reservoirs located in Campo das Vertentes, MG, were sampled in four sampling
    campaigns between 2014 and 2016. A characterization of the systems in their physical and
    chemical aspects (total and dissolved nutrients in the water column, temperature, electrical
    conductance, saturation and concentration of dissolved oxygen, pH, depth of the photic zone)
    and biological (phytoplankton community attributes - composition and total biomass and
    concentration of chlorophyll a in seston), in order to determine the environmental and
    phytoplankton community variables which could be related to the differences in the mineral
    and biochemical composition of seston in the analyzed systems.The characterization of the
    ponds and reservoirs showed great distinction between the eight systems. In the evaluation of
    the internal differences of each system in the mineral and biochemical composition of seston,
    there was practically no spatial variation, both horizontal and vertical, for all variables
    indicators of sestonic nutritional quality. Rare and isolated variations have occurred, which is
    probably related to the small size of the systems.The temporal variation was observed for both
    mineral and biochemical composition in all systems, and mineral composition showed a
    tendency to higher concentrations in the dry season and lower in the transition period. On the other hand, biochemical variables showed higher concentrations in the transition period
    between dry and rainfall. It was not possible to say that one type of sestonic composition
    varied more than the other, so the first hypothesis of this work was not corroborated. In the
    comparison between the systems with different trophic states, significant differences were
    found in both the biochemical and minerals variables. The concentrations of TP and TN in
    seston were higher in the more oligotrophic system, especially in sampling 1, of transition
    between dry and rainfall. The biochemical composition showed a significant difference for the
    lipid concentration, which was lower in the more eutrophic system, more evidenced in the
    samplings 3 and 4. Thus, there were significant differences in both mineral and biochemical
    composition, but they were larger between the two extremes trophic state in the analyzed
    scale. It was not possible to corroborate clearly the second hypothesis. Finally, when the
    responses of the mineral and biochemical composition of seston to environmental variables
    and phytoplankton biomass were compared the relationships between the mineral composition
    and the indicator variables of trophic (dissolved nutrients) and phytoplankton biomass
    (including chlorophyll-a concentration). The responses of the biochemical composition were less significant but had a negative relationship between lipid concentration and trophic
    variables (such as TDN) and biomass (chlorophyll-a), as well as positive relationship between
    seston lipids and the concentration of DO. These results refute the third hypothesis, but
    highlight an important point, which may indicate that the increased trophic state, which is
    usually accompanied by biomass enrichment, may lead to impoverishment of the biochemical
    quality of seston. This reinforces the question of the loss of nutritional quality in aquatic food
    chains due to the eutrophication process, which is a serious problem associated with the
    degradation of surface water nowadays.

2017
Tesis
1
  • JONATAN MARQUES CAMPOS
  • QUANTITATIVE PROTEOMICS OF TROPHOZOITES AND PSEUDOCYSTS OF TRICHOMONAS VAGINALISAND MORPHOLOGICAL ANALYSIS OF THEIR INTERACTION WITH 3D COLLAGEN MATRICES

  • Líder : JOSE BATISTA DE JESUS
  • MIEMBROS DE LA BANCA :
  • MARCOS PAULO CATANHO DE SOUZA
  • ANA CAROLINA RAMOS GUIMARAES
  • LEONARDO SABOIA VAHIA MATILDE
  • JOSE BATISTA DE JESUS
  • LAILA CRISTINA MOREIRA DAMAZIO
  • Data: 31-mar-2017


  • Resumen Espectáculo
  • Recently, our group has demonstrated that iron depletion fromthe culture medium induces the transformation ofT. vaginalistrophozoites into pseudocysts.In the present study,comparativeandquantitative analysesof the proteomesof trophozoites and pseudocysts induced by iron nutritional restrictionwere performed.We also investigated the ability of these two parasitic forms to interact withtype I collagen3D matrices.Differential proteomic analysis oftrophozoites and pseudocysts allowed observationof the protein changes induced upon their interaction dynamics and invasion withtype I collagen matrices.The experimental approaches (LC-MS/MS, GeLC-MS/MS and Label-free shotgun) for sequencing and quantification of soluble, membraneand secreted proteins revealeda drastic decrease in pseudocystsprotein expression when compared to trophozoites.Protein categories exhibitingreduced levelsin pseudocystsweremetabolism, structural, translationand the one assigned as unknown. Thepseudocystsmetabolic proteins with the major alterationsinexpression werePFOR -pyruvate: ferredoxin oxidoreductase BI (-12.00 fold), PFOR -pyruvate: ferredoxin oxidoreductase A (-11.50 fold).Inthesecellularforms,glycolytic pathway proteins were downregulated with the exception of the phosphofructokinase (+2.00 fold) and L-lactate dehydrogenase (+23.50 fold)thatexhibited increased.Thepseudocystsstructural proteins with markeddecreasein expressionwere actinin (-15.00 fold), cofilin (-6.50 fold), Arp2/3 complex, 34kDa(-3.00 fold).The translation machinery of pseudocysts was shown to be compromised by the low expression of60s L6 ribosomal protein (-7.50 fold), leucil tRNA synthetase (-3.00 fold) and L21xiiribosomal protein (-12.00 fold).Proteins involved in cell adhesion processes also showed decreased expression in pseudocystssuch as GP63-like protein (-11.00 fold), AP65-1 (-8.50 fold), AP33-2 (-3.50 fold) and tetraspanin (-3.00 fold).As for the secretomeof trophozoites and pseudocysts,the main molecules identifiedwere cysteine proteases of cathepsin type L-like C1A and endopeptidase-like C13D.In addition, GP63-like type metalloproteases, T-like peptidase M20H and P-like M24G aminopeptidase were found.Interaction analyses of trophozoites and pseudocysts have shown that these two cell forms are capable of interacting, invading and degrading type I collagen matrices.The trophozoites exhibited intense morphological plasticity and a greater adhesion,invasion and degradation capacity on 3D matrices of type I collagen,compared topseudocysts.Altogether, the differential proteomic analysesand the interaction tests with the matrices,allowed us to infer that changes in protein expression may influence how trophozoites and pseudocysts behave duringtheir interaction with the matrices.In this context, we hypothesize that i) thepseudocystsdecreased expression of cytoskeletal and membrane remodeling proteins(Arp2/3 complex, 34kD, actin, proline)may explainthe low morphological plasticity presented by pseudocysts duringthe interaction with the matrices.The trophozoites showed ameboid-like morphologyduringthe interaction with the matrices;ii) the decreased expression of adhesion proteins in pseudocysts (AP65-1, AP33-2, tetraspanin) would explain theirlow adhesion to the collagen matrices; iii) the lower expression of proteolytic enzymes observed in pseudocysts (GP63-like, cathepsin L-like C1A, endopeptidase-like C13D,and oligopeptidase A-like M3A) mayexplain their limited ability to degrade the collagen matricescompared to trophozoites; iv)The large decrease in glycolytic and energetic metabolism proteins indicate that pseudocysts are quiescent forms and resistance to medium variations, similar to that occurring with the resistance forms of other protozoan parasites(Entamoeba histolyticaandGiardia lamblia). These results provide information on the major protein components potentially involved in the interaction of T.vaginaliswith the extracellular matrices, as well as on the biology of pseudocysts.

2
  • GISELE SILVA NOGUEIRA
  • NON-SYNAPTIC CHANGES IN EPILEPSY MODELS OF THETEMPORAL WOLF USING CAINIC ACID

  • Líder : FULVIO ALEXANDRE SCORZA
  • MIEMBROS DE LA BANCA :
  • ANTONIO CARLOS GUIMARAES DE ALMEIDA
  • ANTONIO MAURICIO FERREIRA LEITE MIRANDA DE SA
  • CARLA ALESSANDRA SCORZA BAHI
  • FULVIO ALEXANDRE SCORZA
  • IVAN CARLOS DOS SANTOS
  • Data: 05-abr-2017


  • Resumen Espectáculo
  • Non-synaptic mechanisms play an important role in epileptic seizures. In this work, we
    examined how the nonsynaptic epileptiform activities (NEAS) in the CA1 and dentate gyrus
    (DG) regions of the rat hippocampus were affected by administration of kainate (KA) in two
    different protocols: intraperitoneal (systemic) injection and intra-hippocampal (local) injection.
    The systemic model resulted in changes observed 24h and 7 days after SE, which included
    progressive neuronal loss followed by glial cell proliferation in CA1, CA3 and hilu, DG
    neurogenesis and increased labeling density for NKCC1 in all regions. Non-synaptic ictal
    ++ -K+ activity, induced with the zero-Ca and high protocol, was more pronounced in DG and
    abolished in CA1 in slices of KA groups. Blockage of NKCC1 with bumetanide resulted in
    reduction of NEAS, confirming the influence of this cotransporter on the extracellular potential.
    Although still highly relevant, the effects of systemic application of KA to the hippocampus
    may be influenced by other structures affected by the drug. Thus, in a second moment of the
    work, in order to better understand the direct effects of KA in the hippocampus and to perform
    a comparison between hemispheres, the intra-hippocampal injection model was used. For this
    end, the animals were cannulated and the KA was slowly injected into the right posterior
    hippocampus of animals under waking, without anesthesia effect. Seven days after SE, the
    NEAS were more pronounced in the DG of slices of both hippocampal hemispheres compared
    to the control, although the expression of NKCC1 did not present significant difference. In both
    hemispheres it was identified a higher density interneurons of parvalbumine and Cx36 also in
    DG. A comparison between hemispheres showed significant differences for the DC (greater amplitude) and PS (lower amplitude) parameters of ipsilateral DG, suggesting higher ionic
    movement in this region during NEAS. The ipsilateral hippocampus presented cell death in
    CA1, CA3 and hilu, differently from the contralateral hippocampus. In both, DG cells remained
    preserved. This work allows to conclude that different structures and mechanisms can interfere
    in the electrographic pattern of NEAS. The studies with the two models suggest that the non-
    synaptic changes in the DG contribute to its protagonism in the generation of epileptic seizures
    in the initial phase of epileptogenesis in rats.

3
  • JASIARA CARLA DE OLIVEIRA COELHO
  • INVESTIGATION OF SYNCHRONISM INTRA AND INTER SUBSTRATE AS A MECHANISM OF SEIZURE SUPPRESSION BY SPATIOTEMPORAL VARIATIONS OF ELECTRICAL STIMULATION.

  • Líder : VINICIUS ROSA COTA
  • MIEMBROS DE LA BANCA :
  • CLÁUDIO QUEIROZ
  • DANIEL DE CASTRO MEDEIROS
  • EDUARDO MAZONI ANDRADE MARCAL MENDES
  • LAILA CRISTINA MOREIRA DAMAZIO
  • VINICIUS ROSA COTA
  • Data: 29-nov-2017


  • Resumen Espectáculo
  • In the animal model of seizures induced by pentylenetetrazole (PTZ), electrical stimulation (ES) applied with a random interpulse interval (unstructured / nonperiodic-NPS) to the bilateral amygdala has anticonvulsant properties. According to thenotion that seizures are episodes of neural hypersynchronism, our hypothesis is that NPS may wield its effect by desynchronizing neural networks involved in the ictogenic process.In this sense, the aim of this study is to investigate the hypothesis that nonperiodic electrical stimulation (NPS) acts desynchronizing neural oscillators responsible for ictogenesis. For thisinvestigation, electrophysiological recordings from different epileptogenicmesiotemporal structures of rats, such as the cortex (CX), hippocampus (HP), and thalamus (TH), were performed and non-periodicelectrical stimulation was applied to the bilateral amygdala in animals subjected to the PTZ continuous infusion model.Seven groups were evaluated according to the thresholdsof convulsivant insult and the use or not of NPS. Behavioral and electrophysiological assessments at the neural and network levels were performed to investigate the implications of NPS on hypersynchronism. Electrographic analysis technics evaluated the spike morphology and spikes coincidence between recording channels. Also,phase-amplitude coupling between differentfrequenciesbands was evaluated using the modulation index (MI).Our results corroborate previous studies and the notion that NPS suppressesseizures by a direct desynchronization of epileptogenic networks. Furthermore, additional behavioral findings of groups with different levels of convulsivant insultsuggests NPS acts disturbing transitions between attractors in a state space of seizure susceptibility. Finally, exclusive indings of electrographic tracing studiescontributed to confirm the hypothesis that NPSprobablyacts by desynchronizing neural networks by showing that this temporally unstructured pattern of electrical stimulationis able to promote a decrease in the rate of spikes coincidence between recording channels, as well as to reveal a substantial decrease phase-amplitude coupling between particular neuronal oscillations of frequencies bands of structures involved in ictogenic phenomena.

4
  • SYLVIA ROCHA SILVEIRA PIRES
  • EFFECT OF SOYBEAN PIE ON PERFORMANCE, MILK COMPOSITIONAND PRODUCTION OF ENTERIC METHANE IN DAIRY COWSSÃO JOÃO DEL REIMINAS GERAIS2017

  • Líder : ROGERIO MARTINS MAURICIO
  • MIEMBROS DE LA BANCA :
  • ROGERIO MARTINS MAURICIO
  • ANA PAULA MADUREIRA
  • THIERRY RIBEIRO TOMICH
  • LUIZ GUSTAVO RIBEIRO PEREIRA
  • Adibe Luiz Abdalla
  • Data: 04-dic-2017


  • Resumen Espectáculo
  • Brazil is the second largest soybean producer in the world. Therefore, the coproducts of
    thermic processing for extraction of the oil from soybean grain has potential for dairy
    cows nutrition. Fatty soybean cake (FSC) is a coproduct obtained by the extrusion
    process using Bindgalvão® extruder to obtain oil and FSC. This coproduct, although it is
    a protein source for animal production, it could be used as an alternative to lipid
    supplementation. It is due to the higher concentration of ether extract, compared to
    commercial soybean meal (SBM), which has potential to mitigate enteric CH4 in animal
    production systems. The aims of this study were: i. to evaluate the effect of FSC replaced
    the SBM on the performance of dairy cows and the potential to mitigate enteric CH4; ii.
    to evaluate the facial mask method for measuring CH4 emissions compared to the
    standard method, respirometric chamber. Thirty-two cows (Holandês × Gir) with 112 ±
    25,1 days of lactation, average milk production of 20,8 ± 2,92 Kg/d and 551 ± 45, 4 Kg
    of live weight were used in this experiment. The animals were randomly distributed in 4
    groups by milk production, lactation order and live weight, according to the diet provided
    (0, 6%, 14% and 23% inclusion of FSC in the diet on dry matter base). The diets were
    formulated using LRNS to attend the protein and energy requirements of a 550 Kg cow
    producing an average of 25 Kg/d of milk. The diets were equals in terms of chemical
    composition except for ether extract contents that increased as the proportions of FSC
    increase in the diet. Feed and water intake, live weight and milk production were
    measured automatically during all the experimental period. The apparent digestibility of
    dry matter and nutrients of diets were determined by metabolism test. Blood samples were collected to evaluate the concentrations of non-esterified fatty acids (NEFA), betahydroxybutyrate
    (BHBA), cholesterol, triglycerides and urea in plasma. During the last 4
    weeks of the experimental trial, the CH4 emissions were measured by the respirometric
    chamber and sequentially by the facial mask technique. Samples of ruminal fluid were
    collected by stomach tub to identify populations of methanogenic Archaea. The
    experimental design was a completely randomized and the data were analysed using the
    PROC MIXED SAS. The adjustments between the CH4 emissions using the facial mask
    and respirometric chamber (linear regression, mean bias and correlation coefficient and
    concordance; CCC) were analysed using PROC REG SAS. R and QUIME software were
    used to identify Archaea populations based on microbial DNA data. As increasing the
    FSC levels, a reduction in the production of CH4 (g/Kg DMI and g/Kg milk) was
    observed and the 14% treatment decreased emissions in 11,5 and 20,2% respectively.
    The performance, milk production and ruminal microbial population was not affected by
    FSC. However, the lipid supplementation reduced enteric CH4 emissions. There was no
    difference between the facial mask technique and the respirometric chamber for the
    production of CH4 (g/d, g/Kg MW0,75 and g/Kg milk) but CH4 concentrations (g/Kg
    DMI) were lower using face mask. The linear regression, mean bias and CCC analyses
    demonstrated that the facial mask technique was able to mimic the CH4 emission
    obtained by respirometric chamber. The facial mask has potential to be used in futures in
    vivo studies using a greater number of animals or even treatments. The FSC is an
    alternative to SBM as one strategy to mitigate enteric CH4 emission from dairy cows.

5
  • VICTOR DIEGO CUPERTINO COSTA
  • -

  • MIEMBROS DE LA BANCA :
  • ANTONIO CARLOS GUIMARAES DE ALMEIDA
  • ANTONIO MARCIO RODRIGUES
  • CARLA ALESSANDRA SCORZA BAHI
  • FULVIO ALEXANDRE SCORZA
  • LUIZ EDUARDO CANTON SANTOS
  • MONICA LEVY ANDERSEN
  • Data: 07-dic-2017


  • Resumen Espectáculo
  • -

2016
Disertaciones
1
  • IGOR BARBOSA LIMA
  • CONTAMINANT POTENTIAL OF GLYPHOSATE-BASED HERBICIDES (Roundup®) IN AQUATIC SYSTEMS OF THE RIO DAS MARTES BASIN - MG.

  • Líder : IOLA GONCALVES BOECHAT
  • MIEMBROS DE LA BANCA :
  • BJORN GUCKER
  • CLEBER CUNHA FIGUEREDO
  • IOLA GONCALVES BOECHAT
  • Data: 29-feb-2016


  • Resumen Espectáculo
  • Glyphosate (C3H8NO5P)-based herbicides are known to be associated to several environmental hazards. Thus, the development of efficient and low-cost methodologies for detecting this substance in water samples is crucial to infer its deleterious potential on the structure and functioning of aquatic ecosystems. This work aimed at (1) optimizing the reaction time of glyphosate analysis by automated spectrophotometric flow injection analysis, based on the chromogenic reaction between glyphosate and p-dimethylaminocinnamaldehyde (p-DAC), and (2) testing the optimized method for detecting glyphosate concentrations in water samples from streams submitted to different land uses. The kinetics of the reaction was tested by measuring the absorption of a 5 mg/L glyphosate standard solution after reacting with pDAC color reagent for 60, 120, 240, 480, 960 and 1920 seconds. The p-DAC reaction exhibited saturation kinetics and absorption saturated at approximately 720 s, suggesting that FIA of glyphosate can be optimized by increasing reaction times in the system up to 720 s. The method was also able to detect glyphosate in environmental water samples from streams of different land use with success and proved to be an efficient and low cost method for assessing environmental impacts of glyphosate in aquatic systems. The analysis of water samples collected weekly in streams located in natural, agricultural and urban areas of the Rio das Mortes watershed showed that samples from natural streams were not contaminated with glyphosate based herbicides, whereas streams located in agricultural areas clearly showed glyphosate contamination (concentrations up to 3.64 mg / L ± 0.69). Analyses of samples from urban streams showed the highest levels of glyphosate contamination (concentrations up to 5.61 mg / l ± 0.174). We conclude that mitigation measures are necessary to reduce concentrations of glyphosate in the Rio das Mortes watershed especially in urban areas, in order to avoid damages to both ecosystem structure and functioning.

2
  • LEONARDO JARDIM VAZ DE MELLO
  • NON-SYNAPTIC ACTION OF AMILORIDE IN GERBILES

  • Líder : ANTONIO CARLOS GUIMARAES DE ALMEIDA
  • MIEMBROS DE LA BANCA :
  • ANTONIO CARLOS GUIMARAES DE ALMEIDA
  • CARLA ALESSANDRA SCORZA BAHI
  • FULVIO ALEXANDRE SCORZA
  • Data: 06-dic-2016


  • Resumen Espectáculo
  • Changes in neuronal pH contribute to the onset and development of seizures, and
    changes of 0.5 units or less in this parameter can suppress or trigger paroxysmal
    activity. In general, alkalosis facilitates and acidosis reduces neuronal excitability. For
    this reason, the antiepileptogenic potential of amiloride has been evaluated in animal
    studies, as well as in ex vivo studies in hippocampal slices. The diuretic was the first
    drug identified as a non-specific inhibitor of the sodium/hydrogen exchanger (NHE),
    which, under physiological conditions, performs the antiport between Na+ and H+,
    promoting the influx of Na+ and the efflux of H+, acidifying the extracellular medium.
    Thus, the aim of the present study was to investigate the mechanisms of action of the
    NHE exchanger blocking its effect with amiloride during non-synaptic epileptiform
    activity induced in hippocampal slices of gerbils highly sensitive to the crisis induction
    protocol. The work also involved evaluating the systemic action of the blocker on the
    crises induced in vivo, as well as the effects on the hippocampal structure, in terms of
    the mechanisms of regulation of ionic homeostasis. Highly sensitive to seizure (HSS)
    animals of 14/16 weeks were used in this study. For the selection of these animals, a
    behavioral test was used to induce reflex crises evoked by changes in the
    environment. One week after selection, HSS animals were again stimulated with the
    same protocol and divided into two groups: HSS group with amiloride (HSSA) -
    animals sensitive to reflex crisis stimulus treated with amiloride; and saline sensitive
    group (HSSS) - animals sensitive to stimulus for reflex crisis not treated with
    amiloride. Immediately after the reflex crises followed by the atony period, the
    animals of the HSSA group received amiloride (200 mg /kg) diluted in saline solution
    (0.9%) orally. The animals in the HSSS group received only saline. In each
    investigated group, 31 animals were used, divided for the analysis of behavioral
    assessment (n = 20), electrophysiology (n = 7) and histology (n = 6). The animals
    were decapitated six hours after the administration of amiloride or saline and the induction was performed according to the zero calcium and high potassium model
    with the recording of the extracellular potential (PE) of the hippocampal CA1 region.
    For quantitative analysis of the PE parameters of amplitude of the DC component
    (DC), amplitude of the population spikes (PS’s), duration of events (ED), interval
    between events (TBE) and latency (LT) were analyzed. In immunohistochemical
    analysis, immunostaining for NKCC1, KCC2, Na+/K+ ATPase pump and NHE1 were quantified by optical densitometry. The group of animals treated with amiloride
    exhibited seizures followed by a period of atony with less intensity, as well as a
    smaller number of episodes of myoclonus when compared to the group treated with
    saline. In electrophysiological records, amiloride promoted an important change in
    ictal events that became short (ED reduction), although with an interictal period also
    short, however, without hyper-synchronisms (indicated by large amplitude PSs).
    Since in the ictal period a possible inversion of the NHE exchanger is foreseen,
    treatment with amiloride would help to maintain the extracellular pH, since it would
    prevent the intrusion of H+, besides favoring the increase of the pumping activity by
    Na+/K+-ATPase, due to the accumulation of intracellular Na+, contributing to the end
    of the epileptiform event. The immunohistochemical data evaluated showed no
    change in the expression of the transport mechanisms evaluated, which corroborated
    the electrophysological results. It is necessary to consider the need to continue
    investigating the effects of amiloride in order to identify the portion of that molecule
    responsible for the reduction of paroxysmal activity. This identification should pave
    the way for the development of a multi-acting drug, maximizing antiepileptogenic
    actions and reducing side effects
    Keywords: Gerbils. Amiloride. NHE. Non-synaptic epileptiform activities. Neuronal
    pH.

Tesis
1
  • CASSIA LUANA DE FARIA CASTRO
  • Iron depletion by 2,2-dipyridylinduces the pseudocyst formation, to programmed cell death and modulates Tritrichomonasfoetusplasticity during interaction with 3D collagen I matrices

  • Líder : JOSE BATISTA DE JESUS
  • MIEMBROS DE LA BANCA :
  • MARIA CRISTINA MACHADO MOTTA
  • PATRICIA CUERVO ESCOBAR
  • BJORN GUCKER
  • JOSE BATISTA DE JESUS
  • PRISCILA TOTARELLI MONTEFORTE
  • Data: 04-abr-2016


  • Resumen Espectáculo
  • The extracellular parasite Tritrichomonas foetus(T. foetus)is a protozoan that causes bovine trichomoniasis, a common sexually transmitted disease in cattlewhich may cause losses in livestock due to reproductive failures. The survival of T. foetusduring host-parasite interactionis iron dependent since the metal availability modulates several metabolic pathways of the parasites and regulates the expression of virulence factors such as adhesins and proteolytic enzymes. In this study,the effect of iron depletion onthe morphology and life cycle ofT.foetus, as well as the regulation of the plasticity of the parasites during interaction with 3D collagen I matrices for this metal, was investigated.Scanning and transmission electron microscopy analyses revealed that depletion of iron from the culture medium (named TYM-DIP inducer medium) induces morphological transformation of typical pear-shaped trophozoites into spherical and non-motile pseudocysts.Although iron is required for the survival of T. foetus, iron depletion does not cause a cellular collapse and death throughout the population of parasites, but instead induces cellular changesin pseudocysts, probably in order to allow the parasite to surviveunder conditions ofnutritional stress.In pseudocysts, iron depletion causes the activation of autophagic events that persisting can leadthis population to death by a mechanism that resembles autophagic cell death and not by classical apoptosis. Remarkably, inoculation of xivpseudocysts into iron rich medium (standard TYM medium), or addition of FeSO4to TYM-DIP inducer medium reverted the morphological transformation process and typical trophozoites were recovered, suggesting, that pseudocysts are resistance, viable and reversible forms. It was observed that iron modulates T. foetusplasticity during interaction with 3D collagen I matrices, the two cell forms, typical trophozoites and pseudocysts, recognize and havethe ability to adhere tothematrices. Furthermore, in order to characterize the K strain of T. foetusit was demonstrated that typical trophozoites are able to adhere and phagocyte human vaginal epithelial cells, suggesting that the interaction of this parasite is not species-and cell-especific. Finally, still attempting thecharacterization oftheK strain used in thisstudy, mass spectrometry (LC/MS-MS) coupled to 1D enzimography revealed the profile of cysteine proteases in the total cellular extract and secretome of T. foetus. Among the proteins present in secretion, an important CO involved in the process of the induction of cell death of the host cell and escape of death bythecomplement was also identified.

2
  • ELIANE TRINDADE MARGOTI
  • Analysis of iron effect on cell plasticityof Trichomonas vaginalis

  • Líder : JOSE BATISTA DE JESUS
  • MIEMBROS DE LA BANCA :
  • ANA CAROLINA RAMOS GUIMARAES
  • JOSE BATISTA DE JESUS
  • LAILA CRISTINA MOREIRA DAMAZIO
  • LEONARDO SABOIA VAHIA MATILDE
  • MARCOS PAULO CATANHO DE SOUZA
  • Data: 02-sep-2016


  • Resumen Espectáculo
  • Trichomonas vaginalisis a protozoan parasite of the human urogenital tract responsible for Trichomoniasis Human Urogenital, one of the most common sexually transmitted disease. Iron is an essential element for all cells and bound for survival, proliferation, metabolism, cell plasticity, it alsoregulates virulence factors in tricomononadids. Thethesis aimedto investigate the effect of iron in cellular plasticity and pseudocysts formation inT. vaginalisto verifyifthis morphological ability of the parasite is crucial in the interaction and adherence to human epithelial cells. Thus, it was observed that iron depletion stops theproliferation at different phases of the cell cycle, triggering the morphological transformation of replicative trophozoites to a spherical cell shape nonproliferative, pseudocysts. During the formation of pseudocysts, axostyleand flagella are internalized, being intact flagella involved by membrane into the cytoplasm. This transformation is reversible when the iron is resetin the culture medium. Pseudocysts induced by nutritional stress of iron are forms of resistance that maintain cellular viability, being able to interact and to adhere to human epithelial cells, despite decreaseddramatically his plastic abilitywhen compared to typical trophozoites. The pseudocysts were also identified under natural conditions being detected in fresh clinical specimens from patients with trichomoniasis. The ultrastructural analysis provided a detailed study of external and subcellular morphology of pseudocysts.Thus, our results not only demonstrate the key role of iron in the proliferation and morphology ofthe parasite, but also contributed to a better understanding of the pathogenesis of the parasite mechanisms.

2015
Disertaciones
1
  • Lucas Fernandes Silva
  • DIVERSIDADE MICROBIANA NA ROZOSFERA DE GENÓTIPOS DE MILHO TRANSGÊNICOS EXPRESSANDO O GENE SbMATE CULTIVADOS SOB NÍVEIS CONTRASTANTES DE SATURAÇÃO DE ALUMÍNIO

  • Líder : CLAUDIA TEIXEIRA GUIMARAES
  • MIEMBROS DE LA BANCA :
  • CLAUDIA TEIXEIRA GUIMARAES
  • IVANILDO EVODIO MARRIEL
  • SYLVIA MORAIS DE SOUSA TINOCO
  • Data: 22-abr-2015


  • Resumen Espectáculo
  • DIVERSIDADE MICROBIANA NA ROZOSFERA DE GENÓTIPOS DE MILHO TRANSGÊNICOS EXPRESSANDO O GENE SbMATE CULTIVADOS SOB NÍVEIS CONTRASTANTES DE SATURAÇÃO DE ALUMÍNIO

2014
Disertaciones
1
  • GUSTAVO HENRIQUE ALMEIDA
  • -----

  • Líder : IOLA GONCALVES BOECHAT
  • MIEMBROS DE LA BANCA :
  • BJORN GUCKER
  • CLEBER CUNHA FIGUEREDO
  • IOLA GONCALVES BOECHAT
  • Data: 28-feb-2014


  • Resumen Espectáculo
  • The implementation of aquaculture parks in Brazilian reservoirs has become largely supported by governmental agencies, but requires a previous knowledge of the original conditions of the system, in order to allow the assessment of future impacts in the water quality. Regarding aquaculture impacts, one important variable to be quantified is the relative contribution of organic dissolved nutrients and their eutrophication potential. The aim of this work was to evaluate water and sediment quality of 6 different bays in the Furnas reservoir, where large scale aquaculture is expected to be carried out in the coming years. Special importance was given to the comparison of inorganic and organic fractions of dissolved nitrogen in the water, and as to how those fractions change spatially and temporally in the reservoir. We tested the hypothesis that (1a) concentrations of dissolved organic nitrogen (NOD), although present, do not exceed the concentrations of dissolved inorganic nitrogen (NID) (NOD:NID≤1) and, accordingly, (1b) concentration of particulate inorganic matter (MIP) in sediments should always be higher than concentrations of particulate organic matter (MIP > MOP). We also tested the hypothesis that both spatial (vertical, associated to depth, and horizontal, associated to the distance to reservoir shores) and temporal variation (associated to season) in dissolved inorganic and organic nutrient concentrations occurs. Water and sediment samples were taken in 3 bays belonging to the Sapucaí river and 3 bays belonging to the Grande river. In each bay two sampling sites were selected, one closer to the shore and the second closer to the center of the reservoir. At each sampling station, water samples were taken from the aphotic and the photic zone. In situ measurements of physical and chemical variables were taken at each sapling station andX revealed that DO concentrations were generally lower in Rio Sapucaí bays, whereas specific conductance and the depth of the photic zone were generally smaller in the Rio Grande bays. NOD concentrations were always lower than NID concentrations ( NOD:NID always less than 1). Similarly, MIP concentration always exceeded MOP concentrations in sediments (MIP:MOP>1). Spatial differences in NID concentrations were manly associated with water depth, whereas NOD concentrations were more affected by the distance from shore. This finding points out to a stronger impact of future aquaculture activities on the spatial distribution of organic forms of nutrients and deserves further investigation. Also land use activities seem to already impact NOD concentrations. Vertical distribution of NID may be caused by differences in intake/assimilation and release of inorganic dissolved nitrogen by the plankton community in the photic zone. Concentrations of NOD and NID were generally higher in the warm/rainy season than in the cold/dry season, as a result of the atypical low water level observed during the warm/rainy season of 2012, but did not differ between bays or rivers. Because of the potential of fish-farm activity to impact concentration and distribution of dissolved organic forms of nutrients and MOP in the sediments, these variables should be incorporated in future assessments of aquaculture impacts in Furnas reservoir

2013
Disertaciones
1
  • ANA LÚCIA CARVALHO PEREIRA
  • Manejo da fauna de abelhas pelo plantio associado do manjericão (Ocimum basilicum) e seu papel na produção de frutos e sementes do pimentão

  • Líder : WELLINGTON GARCIA DE CAMPOS
  • MIEMBROS DE LA BANCA :
  • WELLINGTON GARCIA DE CAMPOS
  • LUÍS CLÁUDIO PATERNO SILVEIRA
  • LIVIA MENDES DE CARVALHO SILVA
  • Data: 28-feb-2013


  • Resumen Espectáculo
  • -

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